CDK2 [p Thr160] Antibody

Product Discontinued

CDK2 [p Thr160] Antibody Summary

• Immunogen: Synthetic phosphopeptide derived from human Cdk2 around the phosphorylation site of Threonine 160.
• Modification: p Thr160
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of Cdk2 [p Thr160] pAb in extracts from A2780 cells.
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human, Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CDK2 [p Thr160] Antibody

• cdc2-related protein kinase
• CDK2
• cell devision kinase 2
• Cell division protein kinase 2
• cyclin-dependent kinase 2
• EC 2.7.11
• EC 2.7.11.22
• p33 protein kinase
• p33(CDK2)

Background

Cell cycle progression is dependent on the sequential activation of cyclindependent kinases (Cdks). For full activity, the cell cycle control protein Cdk2 requires phosphorylation of a conserved residue, Threonine 160, carried out by Cdk-activating kinase 1. The kinase associated phosphatase (KAP) is a human dual specificity protein phosphatase that dephosphorylates Cdk2 on Threonine 160 in a cyclin dependent manner. KAP binds to Cdk2 and dephosphorylates Threonine 160 when the associated cyclin subunit is degraded or dissociates. Fluorescence measurements show that Threonine 160 phosphorylation increases the affinity of Cdk2 for both histone substrates and ATP and decreases its affinity for ADP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDK2
• Entrez:
  • 1017(Human)
  • 362817(Rat)