CDK2 Antibody

Product Discontinued

CDK2 Antibody Summary

• Immunogen: Peptide sequence around the aa. 160 (T-Y-T-H-E) from human CDK2 (NP_001789.2).
• Specificity: CDK2 detects endogenous levels of total CDK2 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:1000
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: phosphate buffered saline (without Mg2+and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CDK2 Antibody

• cdc2-related protein kinase
• CDK2
• cell devision kinase 2
• Cell division protein kinase 2
• cyclin-dependent kinase 2
• EC 2.7.11
• EC 2.7.11.22
• p33 protein kinase
• p33(CDK2)

Background

Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDK2 Antibody (NB100-81842)(6)

Publications using NB100-81842

• Park J, Lee J, Yeom Z et al. Neuroprotective effect of Ruminococcus albus on oxidatively stressed SH-SY5Y cells and animals Sci Rep. 2017-11-06 [PMID: 29109537] (WB, Human)
• Hubbi ME, Gilkes DM, Hu H et al. Cyclin-dependent kinases regulate lysosomal degradation of hypoxia-inducible factor 1alpha to promote cell-cycle progression. Proc Natl Acad Sci U S A. 2014-07-28 [PMID: 25071185] (WB, Human, Mouse)
• Liu Y et al. J Biol Chem; 279(6): 4507-14. 2004-01-01 [PMID: 14597612]
• Ukomadu C et al. J Biol Chem; 278(7): 4840-6. 2003-01-01 [PMID: 12475985]
• Morris MC et al. J Biol Chem; 277(26): 23847-53. 2002-01-01 [PMID: 11959850]
• Brown NR et al. J Biol Chem; 274(13): 8746-56. 1999-01-01 [PMID: 10085115]

Review for CDK2 Antibody (NB100-81842) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 10/04/2015

Summary

• Application: Western Blot
• Sample Tested: Human cancer cell
• Species: Human

Bioinformatics

• Gene Symbol: CDK2
• Entrez:
  • 1017(Human)
  • 12566(Mouse)
  • 362817(Rat)
• Uniprot:
  • P24941()