Cdc6 Antibody (DCS-180)

Images

 
Western Blot: Cdc6 Antibody (DCS-180) [NB200-581] - Monoclonal Antibody on MDA231 Cells.
Immunoprecipitation: Cdc6 Antibody (DCS-180) [NB200-581] - Immunoprecipitation of CDC6 using CDC6 Monoclonal Antibody on Native Human Raji Cells.
Immunoprecipitation: Cdc6 Antibody (DCS-180) [NB200-581] - Monoclonal Antibody on denatured Human Raji Cells.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IP
Clone
DCS-180
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Cdc6 Antibody (DCS-180) Summary

Immunogen
Recombinant full length protein (Human).
Localization
The protein is nuclear in G1 and cytoplasmic in S-phase cells
Specificity
Cdc 6 (DCS-180)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CDC6
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2ug / ml
  • Immunoprecipitation 2 ug/ml
Application Notes
Western Blotting: use at a concentration of 1-2ug / ml for 2hrs at RT. Optimal dilutions/concentrations should be determined by the end user. By Western blot, this antibody detects a band of 62 kDa, which corresponds to the predicted molecular weight of Cdc 6.
Reviewed Applications
Read 1 Review rated 5
using
NB200-581 in the following applications:

Publications
Read Publication using NB200-581.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:22223646).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4) and 0.2% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Cdc6 Antibody (DCS-180)

  • CDC18LHsCDC18
  • Cdc18-related protein
  • CDC6 (cell division cycle 6, S. cerevisiae) homolog
  • CDC6 cell division cycle 6 homolog (S. cerevisiae)
  • CDC6 cell division cycle 6 homolog
  • CDC6-related protein
  • cell division control protein 6 homolog
  • cell division cycle 6 homolog (S. cerevisiae)
  • cell division cycle 6 protein
  • HsCdc18
  • HsCDC6
  • p62(cdc6)

Background

The replication licensing system acts to ensure that no section of the genome is replicated more than once in a single cell cycle. The origin recognition complex (ORC) and CDC6/CDC18 are needed on chromatin before the licensing reaction can take place. During the transition from a growth-arrested to a proliferative state, transcription of mammalian CDC6 is regulated by the cell cycle transcription factor (E2F protein). Immunoblots show that minichromosome maintenance (MCM) proteins are not associated with chromatin. Competence of G1 phase nuclei to replicate in vitro coincides with maximum CDC6 accumulation and MCM protein binding to chromatin in vivo. Antibodies against CDC6 and MCM5 stain abnormal (neoplastically transformed) cells in cervical smears and sections with remarkably high specificity and sensitivity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cdc6 Antibody (NB200-581)(1)

Review for Cdc6 Antibody (NB200-581) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB200-581:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Cdc6 NB200-581
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reviewed by:
Anonymous
WB Human 02/18/2011
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Summary

ApplicationWestern Blot
Sample TestedHCT116 Whole Cell Lysate, Sample Amount: 50 ug
SpeciesHuman
CommentsClear detection of endogenous CDC6 from HCT116 cell lysates. Single clear band at expected molecular weight.

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in PBST, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Comments

CommentsClear detection of endogenous CDC6 from HCT116 cell lysates. Single clear band at expected molecular weight.

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Secondary Antibodies

 

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Recent Reviews

5
5
1
4
0
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2
0
1
0

Anonymous
02/18/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol CDC6
Entrez