CDC37 Antibody

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Western Blot: CDC37 Antibody [NB100-92430] - Western blot (WB) analyzes of CDC37 (F259) antibody in extracts from NIH-3T3 cells..

Product Details

Summary
Product Discontinued
View other related CDC37 Primary Antibodies

Order Details


    • Catalog Number
      NB100-92430
    • Availability
      Product Discontinued

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CDC37 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 226-276 of Human Cdc37.
Clonality
Polyclonal
Host
Rabbit
Gene
CDC37
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analyzes of CDC37 (F259) pAb in extracts from NIH-3T3 cells.
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
CDC37 Lysate (NBL1-09002)

Reactivity Notes

This antibody is reactive against Human, Mouse, Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CDC37 Antibody

  • CDC37 (cell division cycle 37, S. cerevisiae, homolog)
  • CDC37 cell division cycle 37 homolog (S. cerevisiae)
  • CDC37 cell division cycle 37 homolog
  • CDC37A
  • cell division cycle 37 homolog (S. cerevisiae)
  • Hsp90 chaperone protein kinase-targeting subunit
  • hsp90 co-chaperone Cdc37
  • P50CDC37

Background

Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with HSP 90. Cdc34, Cdc27 and Cdc16 function as ubiquitinconjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphasepromoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CDC37
Entrez