Cdc27 Antibody

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Western Blot: Cdc27 Antibody [NB100-59832] - Detection of Human CDC27 on HeLa whole cell lysate using NB100-59832. CDC27 was also immunoprecipitated by rabbit anti-CDC27 antibody NB100-59831.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-59832
    • Availability
      Product Discontinued

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Cdc27 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 774 and 824 of human cell division cycle 27 using the numbering given in entry NP_001247.2 (GeneID 996).
Clonality
Polyclonal
Host
Rabbit
Gene
CDC27
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 1:10-1:500
Positive Control
Cdc27 Lysate (NBL1-08998)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cdc27 Antibody

  • ANAPC3CDC27 homolog
  • anaphase promoting complex subunit 3
  • Anaphase-promoting complex subunit 3
  • anaphase-promoting complex, protein 3
  • APC 3
  • APC3cell division cycle 27
  • CDC27Hs
  • cell division cycle 27 homolog (S. cerevisiae)
  • cell division cycle protein 27 homolog
  • D0S1430E
  • D17S978E
  • HNUC
  • H-NUC
  • nuc2 homolog
  • NUC2

Background

CDC27 is a component of the APC/c (anaphase promoting complex/cyclosome) which is responsible for the ubiquitination and degradation of securin and cyclin B that prompts the onset of anaphase and exit from mitosis. The APC/c is composed of at least 11 subunits. Three subunits, CDC27 CDC16 and CDC23, contain a TPR (tetratricopeptide repeat) important for protein-protein interactions. CDC27 has been shown to interact with mitotic checkpoint proteins such as Mad2, p55CDC and BUBR1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Cdc27 Antibody (NB100-59832)

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Bioinformatics

Gene Symbol CDC27
Entrez
Uniprot