CDC25A Antibody (DCS-121)

Images

 
Western Blot: Cdc25A Antibody (DCS-121) [NBP1-54460] - Western blot analysis of CDC25A expression in Hela cells (lane 1) NIH/3T3 cells (lane 2) and Rat-1 cells (lane3)
Immunohistochemistry-Paraffin: Cdc25A Antibody (DCS-121) [NBP1-54460] - detection of CDC25A on paraffin embedded section of human tonsil (germinal center).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clone
DCS-121
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CDC25A Antibody (DCS-121) Summary

Immunogen
The immunogen for this antibody is CDC25A. The exact immunogenic sequence is proprietary.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CDC25A
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-5 ug/ml
  • Immunohistochemistry 1-5 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Reactivity Notes

Human, Mouse, Rat. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for CDC25A Antibody (DCS-121)

  • CDC25A
  • CDC25A2
  • CDC25A2-CAG isoform
  • cell division cycle 25 homolog A (S. cerevisiae)
  • cell division cycle 25 homolog A (S. pombe)
  • cell division cycle 25A
  • Dual specificity phosphatase Cdc25A
  • EC 3.1.3.48
  • M-phase inducer phosphatase 1

Background

The Cdc2/cyclin B enzyme, involved in regulation of mitosis in eukaryotic cells,is subject to multiple levels of control. Among these, the regulation of the catalytic subunit by Tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B complex, while tyrosine dephosphorylation,which occurs at the onset of mitosis, directly activates the pre-MPH complex. The Cdc25 gene serves as a rate-limiting mitotic activator, apparently due to its action as the Cdc2 tyrosine phosphatase. In the absence of Cdc25,Cdc2 accumulates in a tyrosine phosphorylated state. In addition, Cdc25 proteins from a variety of species have been shown to share a low degree of sequence similarity with other tyrosine phosphatases. The Cdc25 gene family consists of at least three members that share approximately 40% identity in their most conserved carboxy-terminal sequences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CDC25A
Entrez