Cdc23 Antibody (NB100-78338)

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Western Blot: Cdc23 Antibody [NB100-78338] - la nuclear cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-APC8 antibody. Proteins were visualized using a donkey anti-rabbit ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-78338
    • Availability
      Product Discontinued

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Cdc23 Antibody Summary

Immunogen
Recombinant (partial), N-terminal
Specificity
APC8
Clonality
Polyclonal
Host
Rabbit
Gene
CDC23
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 mul per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Positive Control
Cdc23 Lysate (NBL1-08993)

Reactivity Notes

Mouse, Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Cdc23 Antibody

  • ANAPC8anaphase promoting complex subunit 8
  • Anaphase-promoting complex subunit 8
  • APC8CDC23 (cell division cycle 23, yeast, homolog)
  • cell division cycle 23 homolog (S. cerevisiae)
  • cell division cycle protein 23 homolog
  • CUT23
  • Cyclosome subunit 8

Background

APC8 (anaphase-promoting complex subunit 8) is a member of the E3 enzyme family. This protein contains TPR repeats and has a molecular weight of approximately 68 kD. The APC8 protein is located in the nucleus during interphase and the centrosome during metaphase/anaphase. This protein is required for mitotic anaphase transition. The APC8 protein functions with other members of the APC complex as a multisubunit cell cycle ubiquitin ligase, and a regulator of sister chromatid separation by degrading securins. In addition, this protein functions in ubiquitin-dependent cyclin catabolism, metaphase/anaphase transition, and spindle elongation. The APC8 protein comprises one subunit of the anaphase promoting complex including APC1-8, and other probable complex proteins APC9-11, Cdc26, Mnd2, Swm1. The APC complex is inactivated by protein kinase A and is activated by CDC20 and Cdh1. APC8 is phosphorylated by cdk1-cyclinB and PLK-1. The Poly6114 antibody has been shown to be useful for Western blotting of the human and mouse APC8 protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CDC23
Entrez