Cdc20 Antibody

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-78051
    • Availability
      Product Discontinued

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Cdc20 Antibody Summary

Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 486-499 of Human Cdc20 (fizzy) (C-terminal) coupled to KLH.
Specificity
This product reacts with human CDC20 (fizzy). Cross reactivity may also occur with CDC20 from other sources.
Isotype
Serum
Clonality
Polyclonal
Host
Rabbit
Gene
CDC20
Purity
Sterile filtered
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA 1:2000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates. Coimmunoprecipitation of related proteins has not been determined. A 54.7 kDa band corresponding - human CDC20 is detected. Most cell lines or tissues expressing CDC20 can be used as a positive control. Researchers should determine optimal titers for other applications.

Reactivity Notes

Cross reactivity against Cdc20 from other tissues and species may occur but has not been specifically tested.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Antiserum
Preservative
0.01% Sodium Azide
Concentration
85 mg/ml
Purity
Sterile filtered

Alternate Names for Cdc20 Antibody

  • CDC20 cell division cycle 20 homolog (S. cerevisiae)
  • CDC20 cell division cycle 20 homolog
  • cell division cycle 20 homolog (S. cerevisiae)
  • cell division cycle protein 20 homolog
  • MGC102824
  • p55CDCS. cerevisiae, homolog)

Background

CDC20, also known as fizzy, Cell division cycle protein 20 homolog, and p55CDC is required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. CDC20 is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The phosphorylated form of CDC20 interacts with APC/C. Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CDC20