CDC2/CDK1 [p Tyr15] Antibody

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Western Blot: CDC2/CDK1 [p Tyr15] Antibody [NBP1-51453] - Analysis of p-Cdk1/Cdc2 (Y15) pAb at 1:500 dilution Lane1:HEK293T cell lysate treated with colchicine Lane2:sp2/0 cell lysate treated with colchicine Lane3:PC12 ...read more
Western Blot: CDC2/CDK1 [p Tyr15] Antibody [NBP1-51453] - Extracts from HepG2 cells.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-51453
    • Availability
      Product Discontinued

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CDC2/CDK1 [p Tyr15] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human Cdk1/Cdc2 around the phosphorylation site of Serine 795.
Modification
p Tyr15
Specificity
p-Cdc2 (Y15) pAb detects endogenous levels of Cdc2 protein only when phosphorylated at Y15.
Clonality
Polyclonal
Host
Rabbit
Gene
CDK1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
Application Notes
Western blot (WB) analyzes of p-Cdc2 (Y15) pAb in extracts from HepG2 cells.
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CDC2/CDK1 [p Tyr15] Antibody

  • CDC2
  • CDC28A
  • CDC2MGC111195
  • CDK1
  • cell cycle controller CDC2
  • Cell division control protein 2 homolog
  • Cell division protein kinase 1
  • cyclin-dependent kinase 1
  • DKFZp686L20222
  • EC 2.7.11.22
  • EC 2.7.11.23
  • G1 to S and G2 to M
  • p34 protein kinase
  • P34CDC2

Background

Cdc2, an evolutionarily conserved serine/threonine-specific protein kinase, is essential in the cell cycle transition from G2 to M phase. Cdc2 is regulated by association with B-type cyclins and by reversible phosphorylation. Cyclin B binding facilitates the phosphorylation of Cdc2 p34 on three regulatory sites: Threonine 14, Tyrosine 15 and Threonine 161. In higher eukaryotes, Cdc2 is negatively regulated by phosphorylation of two residues located in the ATP-binding site, Thr 14 and Tyr 15. Cdc2 is positively regulated by the cyclindependent phosphorylation of Thr 161. Both phosphorylation and dephosphorylation at Thr 161 are required for progression through the cell cycle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CDK1
Entrez