CD8 beta Antibody (341)

Images

 
Flow Cytometry: CD8 beta Antibody (341) [NBP1-43450] - Analysis using the FITC conjugate of NBP1-43450. Staining of rat thymocytes with 0.25 ug of Mouse IgG1 kappa Isotype Control FITC (open histogram) or 0.25 ug of ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, Flow, Func, IHC, IHC-P, IP
Clone
341
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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Catalog# & Conjugate Size Price

CD8 beta Antibody (341) Summary

Immunogen
The immunogen for this antibody was CD8b.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD8B
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Functional
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Reactivity Notes

This antibody is reactive to Rat.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD8 beta Antibody (341)

  • CD8 antigen, beta polypeptide 1 (p37)
  • CD8 beta
  • CD8b antigen
  • CD8b molecule
  • CD8b
  • CD8B1
  • CD8B1P37
  • Leu2
  • LY3
  • LYT3
  • MGC119115
  • P37
  • T lymphocyte surface glycoprotein beta chain
  • T-cell surface glycoprotein CD8 beta chain

Background

The 341 monoclonal antibody reacts with rat CD8beta. CD8beta is a member of the immunoglobulin superfamily which heterodimerizes with CD8alpha to form the CD8 antigen expressed on CD4+CD8+, double-positive thymocytes, the majority of MHC class I-restricted peripheral T cells, and on some macrophages. CD8beta expression is absent in athymic mice, which are populated by T-cells expressing CD8alpha homodimers. This has led to the idea that CD8beta expression is dependent on the development of T cells in the thymus. CD8 binds to MHC class I expressed on the surface of antigen-presenting cells during antigen presentation, and participates in T-cell receptor signal transduction through association with the kinase Lck.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NBP1-43450APC
FITC Labeled NBP1-44039

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Bioinformatics

Gene Symbol CD8B
Entrez