CD8 alpha Antibody (YTS105.18)


Immunocytochemistry/ Immunofluorescence: CD8 alpha Antibody (YTS105.18) [NB200-578] - Staining of a mouse lymph node cryosection with Rat anti mouse CD19, clone 6D5 (MCA1439), green in A and Rat anti Mouse CD8, red in more
Immunohistochemistry-Frozen: CD8 alpha Antibody (YTS105.18) [NB200-578] - Published customer Image. Staining of (A) Infiltration of NKG2D-positive cells into tumors. Northern blot analysis was performed to detect NKG2D more
Flow Cytometry: CD8 alpha Antibody (YTS105.18) [NB200-578] - Staining of mouse spleen cells.
Immunohistochemistry: CD8 alpha Antibody (YTS105.18) [NB200-578] - Staining of Mouse lymph node cryosection.

Product Details

Reactivity Hu, MuSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr
1.0 mg/ml

CD8 alpha Antibody (YTS105.18) Summary

Mouse spleen cells
Cell Membrane and Secreted
NB200-578 recognises a non polymorphic epitope on the mouse CD8 alpha chain. Blocks MHC I dependent T cell responses in vitro and in vivo, and induces transplantation tolerance in combination with CD4 antibodies.
IgG2 Alpha
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. IHC-P: It has been reported that the antibody did not work in paraffin embedded mouse spleen tissue.
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NB200-578.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Phosphate buffered saline
0.09% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for CD8 alpha Antibody (YTS105.18)

  • CD8 antigen, alpha polypeptide (p32)
  • CD8
  • CD8a antigen
  • CD8a molecule
  • Leu2 T-lymphocyte antigen
  • Leu2
  • MAL
  • OKT8 T-cell antigen
  • p32
  • RPA-T8 antibody flow
  • RPA-T8 Antibody
  • RPA-T8 CD8
  • RPA-T8 Clone
  • RPA-T8 Flow
  • T cell co-receptor
  • T8 T-cell antigen
  • T-cell antigen Leu2
  • T-cell surface glycoprotein CD8 alpha chain
  • T-lymphocyte differentiation antigen T8/Leu-2




This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: Flow, ICC/IF, IHC, IHC-Fr

Publications for CD8 alpha Antibody (NB200-578)(9)

Showing Publications 1 - 9 of 9.
Publications using NB200-578 Applications Species
Nakashima, H et al. A Novel Combination Immunotherapy for Cancer by IL-13Ra2-Targeted DNA Vaccine and Immunotoxin in Murine Tumor Models. J Immunol 187: 4935-46. 2011 [PMID: 22013118]
Lacroix-Lamande, S et al. Neonate intestinal immune response to CpG oligodeoxynucleotide stimulation. PLoS One 4: 1-8. 2009 [PMID: 20011519]
Karlsson, MR et al. Hypersensitivity and oral tolerance in the absence of a secretory immune system. Allergy 65: 561-70. 2010 [PMID: 19886928]
Auray, G et al. (2007) Involvement of intestinal epithelial cells in dendritic cell recruitment during C. parvum infection Microbes Infect 9: 574-82. 2007 [PMID: 17395519]
Himoudi, N et al. (2007) Development of anti-PAX3 immune responses; a target for cancer immunotherapy. Cancer Immunol Immunother 56: 1381-95. 2007 [PMID: 17318653]
Sroga, JM et al. Rats and mice exhibit distinct inflammatory reactions after spinal cord injury. J Comp Neurol. 462: 223-40. 2003 [PMID: 12794745]
Wise, MP et al. Cutting edge: Linked suppression of skin graft rejection can operate through indirect recognition. J Immunol 161: 5813-5816. 1998 [PMID: 9834057]
Cobbold, SP et al. The induction of skin graft tolerance in MHC-mis matched or primed recipients: primed T-cells can be tolerized in the periphery with CD4 and CD8 antibodies. Eur. J. Immunol. 20: 2747-2755. 1990 [PMID: 1980112]
Qin, S et al. (1990) Induction of tolerance in peripheral T cells with monoclonal antibodies. Eur. J. Immunol. 20: 2737-2745. 1990 [PMID: 1702726]

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for CD8 alpha Antibody (NB200-578). (Showing 1 - 6 of 6 FAQs).

  1. I'm looking for a good anti CD8  antibody working on immunofluorescence on murine tumoral tissue fixed in paraformaldehyde, cryoprotected with sucrose, frozen in OCT medium and sectioned with a cryostat.
    • I would recommend our CD8 alpha antibody NBP1-49045, it is guaranteed to work in IHC-P and and IHC-FR.   
  2. How do I know if this will actually work in IHC?
    • This CD8 alpha antibody has been validated in IHC and has been cited in a paper where it was used in IHC.
  3. Will this CD8 alpha antibody work for rabbit in IHC-FR?
    • Unfortunately this CD8 alpha antibody has not yet been validated in rabbit and there no sequence info in uniprot yet so we can't speculate based on homology. You may want to take advantage of our innovator's reward program which will allow you to test in rabbit without any financial risk. 
  4. Does this CD8 alpha antibody detect both forms of CD8?
    • Both the alpha - beta heterodimer as well as the alpha - alpha homodimer are detected by the (53-6.7) CD8 alpha antibody.
  5. Would you recommend your CD8 alpha antibody as a T-Cell marker? 
    • Yes this CD8 alpha anitbody has been cited in a study where it was used in FACS to separate out CD8+ T-cells. Please see the references tab PMID: 1608966. It is important to note that our CD8 alpha antibody may also stain mature dendritic cells and NK cells.
  6. May we ask the suggested dilution titers while using NB200-578 and NBP1-43449 for IHC?
    • A good starting point for optimization in IHC for both products would be 1:100.

Secondary Antibodies


Isotype Controls

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CD8 alpha - Marker for cytotoxic T lymphocytes
The T-cell co-receptor CD8 is a cell-surface glycoprotein that bridges the lipid bilayer by interacting externally with ligands, such as major histocompatibility complex (MHC) class I, as well as internally with signaling molecules such as tyrosine ki...  Read full blog post.

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Gene Symbol CD8A