CD8 alpha Antibody (RPA-T8) [Allophycocyanin]

Images

 
Flow Cytometry: CD8 alpha Antibody (RPA-T8) [Allophycocyanin] [NBP2-27246] - Cell surface flow cytometric analysis of CD8 in 1x106 human PBMC using 10 ul (0.1 ug) of IMG-5917G. Green represents isotype control (IMGENEX, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
RPA-T8
Clonality
Monoclonal
Host
Mouse
Conjugate
Allophycocyanin

CD8 alpha Antibody (RPA-T8) [Allophycocyanin] Summary

Immunogen
CD8 (CD8a; alpha chain)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD8A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1ul/1 million cells
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This product reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for CD8 alpha Antibody (RPA-T8) [Allophycocyanin]

  • CD8 antigen, alpha polypeptide (p32)
  • CD8
  • CD8a antigen
  • CD8a molecule
  • Leu2 T-lymphocyte antigen
  • Leu2
  • MAL
  • OKT8 T-cell antigen
  • p32
  • RPA-T8 antibody flow
  • RPA-T8 Antibody
  • RPA-T8 CD8
  • RPA-T8 Clone
  • RPA-T8 Flow
  • T cell co-receptor
  • T8 T-cell antigen
  • T-cell antigen Leu2
  • T-cell surface glycoprotein CD8 alpha chain
  • T-lymphocyte differentiation antigen T8/Leu-2

Background

CD8 is acell surface glycoprotein expressing on thymocytes subsets and cytotoxic T cells and is either a homodimer of two alpha chains, or a heterodimer of one alpha and one beta chain. It acts as a coreceptor during T cell activation through the binding of MHC Class I molecules and is found on T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 associates with LCk with the help of a zinc clasp structure and down-regulates the production of major Th2-type cytokines. CD8 is also known to recruit pUL97 thereby inducing dissolution of the nuclear lamina and facilitating the nuclear export of viral capsids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD8 alpha Antibody (NBP2-27246). (Showing 1 - 6 of 6 FAQ).

  1. I'm looking for a good anti CD8  antibody working on immunofluorescence on murine tumoral tissue fixed in paraformaldehyde, cryoprotected with sucrose, frozen in OCT medium and sectioned with a cryostat.
    • I would recommend our CD8 alpha antibody NBP1-49045, it is guaranteed to work in IHC-P and and IHC-FR.   
  2. How do I know if this will actually work in IHC?
    • This CD8 alpha antibody has been validated in IHC and has been cited in a paper where it was used in IHC. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3387923/
  3. Will this CD8 alpha antibody work for rabbit in IHC-FR?
    • Unfortunately this CD8 alpha antibody has not yet been validated in rabbit and there no sequence info in uniprot yet so we can't speculate based on homology. You may want to take advantage of our innovator's reward program which will allow you to test in rabbit without any financial risk. 
  4. Does this CD8 alpha antibody detect both forms of CD8?
    • Both the alpha - beta heterodimer as well as the alpha - alpha homodimer are detected by the (53-6.7) CD8 alpha antibody.
  5. Would you recommend your CD8 alpha antibody as a T-Cell marker? 
    • Yes this CD8 alpha anitbody has been cited in a study where it was used in FACS to separate out CD8+ T-cells. Please see the references tab PMID: 1608966. It is important to note that our CD8 alpha antibody may also stain mature dendritic cells and NK cells.
  6. The vial for this product is lacking in size and concentration information.
    • The 100 Test size vial should contain 1.0ml at 0.01mg/ml. Our recommended dilution for use is 10ul/1 x 10^6 cells (0.1ug).

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 350 Labeled NBP2-25195UV
DyLight 405 Labeled NBP2-25195V
DyLight 488 Labeled NBP2-25195G
DyLight 550 Labeled NBP2-25195R
DyLight 650 Labeled NBP2-25195C
DyLight 680 Labeled NBP2-25195FR
DyLight 755 Labeled NBP2-25195IR
HRP Labeled NBP2-25195H
PE Labeled NBP2-27237
PerCP Labeled NBP2-25195PCP

Additional Array Products

Bioinformatics Tool for CD8 alpha Antibody (NBP2-27246)

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Research Areas for CD8 alpha Antibody (NBP2-27246)

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Blogs on CD8 alpha.

CD8 alpha - Marker for cytotoxic T lymphocytes
The T-cell co-receptor CD8 is a cell-surface glycoprotein that bridges the lipid bilayer by interacting externally with ligands, such as major histocompatibility complex (MHC) class I, as well as internally with signaling molecules such as tyrosine ki...  Read full blog post.

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Bioinformatics

Gene Symbol CD8A