CD8 alpha Antibody (RFT-8) [PE]

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Flow Cytometry: CD8 alpha Antibody (RFT-8) [PE] [NBP1-28421] - Analysis of peripheral blood lymphocytes by multiple staining.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-28421
    • Availability
      Product Discontinued

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CD8 alpha Antibody (RFT-8) [PE] Summary

Immunogen
Thymocytes and E-rosetted lymphocytes
Specificity
Human CD8
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD8A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS and a stabilizing agent
Preservative
Sodium Azide
Purity
Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for CD8 alpha Antibody (RFT-8) [PE]

  • CD8 antigen, alpha polypeptide (p32)
  • CD8
  • CD8a antigen
  • CD8a molecule
  • Leu2 T-lymphocyte antigen
  • Leu2
  • MAL
  • OKT8 T-cell antigen
  • p32
  • RPA-T8 antibody flow
  • RPA-T8 Antibody
  • RPA-T8 CD8
  • RPA-T8 Clone
  • RPA-T8 Flow
  • T cell co-receptor
  • T8 T-cell antigen
  • T-cell antigen Leu2
  • T-cell surface glycoprotein CD8 alpha chain
  • T-lymphocyte differentiation antigen T8/Leu-2

Background

CD8 is a 32 kDa transmembrane glycoprotein expressed as either a CD8alpha alpha homodimer or CD8alpha beta heterodimer. It is expressed on the 'cytotoxic/suppressor' subpopulation of peripheral T cells. CD8 functions primarily as a coreceptor with MHC Class I-restricted TCR's in antigen recognition. (1-2)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD8 alpha Antibody (NBP1-28421) (0)

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Product General Protocols

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FAQs for CD8 alpha Antibody (NBP1-28421). (Showing 1 - 5 of 5 FAQ).

  1. I'm looking for a good anti CD8  antibody working on immunofluorescence on murine tumoral tissue fixed in paraformaldehyde, cryoprotected with sucrose, frozen in OCT medium and sectioned with a cryostat.
    • I would recommend our CD8 alpha antibody NBP1-49045, it is guaranteed to work in IHC-P and and IHC-FR.   
  2. How do I know if this will actually work in IHC?
    • This CD8 alpha antibody has been validated in IHC and has been cited in a paper where it was used in IHC. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3387923/
  3. Will this CD8 alpha antibody work for rabbit in IHC-FR?
    • Unfortunately this CD8 alpha antibody has not yet been validated in rabbit and there no sequence info in uniprot yet so we can't speculate based on homology. You may want to take advantage of our innovator's reward program which will allow you to test in rabbit without any financial risk. 
  4. Does this CD8 alpha antibody detect both forms of CD8?
    • Both the alpha - beta heterodimer as well as the alpha - alpha homodimer are detected by the (53-6.7) CD8 alpha antibody.
  5. Would you recommend your CD8 alpha antibody as a T-Cell marker? 
    • Yes this CD8 alpha anitbody has been cited in a study where it was used in FACS to separate out CD8+ T-cells. Please see the references tab PMID: 1608966. It is important to note that our CD8 alpha antibody may also stain mature dendritic cells and NK cells.

Secondary Antibodies

 

Isotype Controls

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Research Areas for CD8 alpha Antibody (NBP1-28421)

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Blogs on CD8 alpha.

CD8 alpha - Marker for cytotoxic T lymphocytes
The T-cell co-receptor CD8 is a cell-surface glycoprotein that bridges the lipid bilayer by interacting externally with ligands, such as major histocompatibility complex (MHC) class I, as well as internally with signaling molecules such as tyrosine ki...  Read full blog post.

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Bioinformatics

Gene Symbol CD8A