CD8 alpha Antibody Pair

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal

Order Details

CD8 alpha Antibody Pair Summary

Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.

  • Antibody pair for IP: mouse monoclonal anti-CD8A (300 ug)
  • Antibody pair for WB: rabbit polyclonal anti-CD8A (50 ul)
  • Clonality
    Polyclonal
    Gene
    CD8A
    Purity
    Immunogen affinity purified

    Packaging, Storage & Formulations

    Storage
    Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
    Preservative
    No Preservative
    Purity
    Immunogen affinity purified

    Notes

    Quality control test: Immunoprecipitation-Western Blot (IP-WB).

    This product is produced by and distributed for Abnova, a company based in Taiwan.

    Alternate Names for CD8 alpha Antibody Pair

    • CD8 antigen, alpha polypeptide (p32)
    • CD8
    • CD8a antigen
    • CD8a molecule
    • Leu2 T-lymphocyte antigen
    • Leu2
    • MAL
    • OKT8 T-cell antigen
    • p32
    • RPA-T8 antibody flow
    • RPA-T8 Antibody
    • RPA-T8 CD8
    • RPA-T8 Clone
    • RPA-T8 Flow
    • T cell co-receptor
    • T8 T-cell antigen
    • T-cell antigen Leu2
    • T-cell surface glycoprotein CD8 alpha chain
    • T-lymphocyte differentiation antigen T8/Leu-2

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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    Product General Protocols

    Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

    Video Protocols

    WB Video Protocol

    FAQs for CD8 alpha Antibody Pair (H00000925-PW1). (Showing 1 - 5 of 5 FAQ).

    1. I'm looking for a good anti CD8  antibody working on immunofluorescence on murine tumoral tissue fixed in paraformaldehyde, cryoprotected with sucrose, frozen in OCT medium and sectioned with a cryostat.
      • I would recommend our CD8 alpha antibody NBP1-49045, it is guaranteed to work in IHC-P and and IHC-FR.   
    2. How do I know if this will actually work in IHC?
      • This CD8 alpha antibody has been validated in IHC and has been cited in a paper where it was used in IHC. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3387923/
    3. Will this CD8 alpha antibody work for rabbit in IHC-FR?
      • Unfortunately this CD8 alpha antibody has not yet been validated in rabbit and there no sequence info in uniprot yet so we can't speculate based on homology. You may want to take advantage of our innovator's reward program which will allow you to test in rabbit without any financial risk. 
    4. Does this CD8 alpha antibody detect both forms of CD8?
      • Both the alpha - beta heterodimer as well as the alpha - alpha homodimer are detected by the (53-6.7) CD8 alpha antibody.
    5. Would you recommend your CD8 alpha antibody as a T-Cell marker? 
      • Yes this CD8 alpha anitbody has been cited in a study where it was used in FACS to separate out CD8+ T-cells. Please see the references tab PMID: 1608966. It is important to note that our CD8 alpha antibody may also stain mature dendritic cells and NK cells.

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    Blogs on CD8 alpha.

    CD8 alpha - Marker for cytotoxic T lymphocytes
    The T-cell co-receptor CD8 is a cell-surface glycoprotein that bridges the lipid bilayer by interacting externally with ligands, such as major histocompatibility complex (MHC) class I, as well as internally with signaling molecules such as tyrosine ki...  Read full blog post.

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    Bioinformatics

    Gene Symbol CD8A