CD8 alpha Antibody (LT8)


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Product Details

Reactivity HuSpecies Glossary
Applications WB, B/N
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Order Details

CD8 alpha Antibody (LT8) Summary

Normal human blood lymphocytes.
Type I membrane protein (isoform 1) and secreted (isoform 2).
Reacts with CD8 antigen (MW 32 kDa). NB110-16584 completely cross blocks binding of MAbs Leu 2a and OKT8.
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS. pH 7.4
0.09% Sodium Azide
Protein A or G purified


  • Western Blot 1:5000
  • Blocking/Neutralizing 50-200 molar excess
Application Notes
Can be used to enumerate T suppressor/cytotoxic subset in peripheral blood and tissue.
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Alternate Names for CD8 alpha Antibody (LT8)

  • CD8 antigen, alpha polypeptide (p32)
  • CD8
  • CD8a antigen
  • CD8a molecule
  • Leu2 T-lymphocyte antigen
  • Leu2
  • MAL
  • OKT8 T-cell antigen
  • p32
  • RPA-T8 antibody flow
  • RPA-T8 Antibody
  • RPA-T8 CD8
  • RPA-T8 Clone
  • RPA-T8 Flow
  • T cell co-receptor
  • T8 T-cell antigen
  • T-cell antigen Leu2
  • T-cell surface glycoprotein CD8 alpha chain
  • T-lymphocyte differentiation antigen T8/Leu-2


Hybridoma clone (LT8) has been derived from hybridization of X63.653 myeloma with spleen cells of Balb/c mice immunized with normal human blood lymphocytes. CD8 is present on 23 +/- 4 % of normal peripheral blood lymphocytes and 80 % of thymocytes. This antigen is found on T suppressor/cytotoxic T cells


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CD8 alpha Antibody (NB110-16584). (Showing 1 - 5 of 5 FAQ).

  1. I'm looking for a good anti CD8  antibody working on immunofluorescence on murine tumoral tissue fixed in paraformaldehyde, cryoprotected with sucrose, frozen in OCT medium and sectioned with a cryostat.
    • I would recommend our CD8 alpha antibody NBP1-49045, it is guaranteed to work in IHC-P and and IHC-FR.   
  2. How do I know if this will actually work in IHC?
    • This CD8 alpha antibody has been validated in IHC and has been cited in a paper where it was used in IHC.
  3. Will this CD8 alpha antibody work for rabbit in IHC-FR?
    • Unfortunately this CD8 alpha antibody has not yet been validated in rabbit and there no sequence info in uniprot yet so we can't speculate based on homology. You may want to take advantage of our innovator's reward program which will allow you to test in rabbit without any financial risk. 
  4. Does this CD8 alpha antibody detect both forms of CD8?
    • Both the alpha - beta heterodimer as well as the alpha - alpha homodimer are detected by the (53-6.7) CD8 alpha antibody.
  5. Would you recommend your CD8 alpha antibody as a T-Cell marker? 
    • Yes this CD8 alpha anitbody has been cited in a study where it was used in FACS to separate out CD8+ T-cells. Please see the references tab PMID: 1608966. It is important to note that our CD8 alpha antibody may also stain mature dendritic cells and NK cells.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

PE Labeled NB100-64029

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Blogs on CD8 alpha.

CD8 alpha - Marker for cytotoxic T lymphocytes
The T-cell co-receptor CD8 is a cell-surface glycoprotein that bridges the lipid bilayer by interacting externally with ligands, such as major histocompatibility complex (MHC) class I, as well as internally with signaling molecules such as tyrosine ki...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol CD8A

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