CD8 alpha Antibody (FK18)



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CD8 alpha Antibody (FK18) Summary

The antibody reacts with CD8, the 33 kD T8 antigen present on the human cytotox­ic/suppressor T-cell subset.
The antibody reacts with CD8, the 33 kD T8 antigen present on the human cytotox­ic/suppressor T-cell subset. It strongly blocks the cytolytic activity of T8-positive CTLs. It also reacts with a subpopulation of Rhesus monkey lymphocytes which is identical to the population of human T8 positive cells.
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
No buffer
Sodium Azide
Protein A or G purified


Application Notes
The antibody can be used for the identification of CD8-positi­ve cells using immuno­flu­orescence or immunoperoxidase procedu­res and for the removal of CD8 positive cells using complement dependent cytotoxicity. In addition the antibody blocks the cytotoFor use in regular immunofluorescence and microcytotoxicity methods, dilution of 1:10, preferably in phosphate buffered saline plus 1% BSA and 0.02% sodium azide is advised. See for advised methods instructions A and B.

Reactivity Notes



CD8a is a 32 kD glycoprotein also known as T8, Lyt2, Ly-2, and CD8 alpha. CD8a is a member of the immunoglobulin superfamily expressed on most thymocytes, subset of mature T cells, most NK cells, macrophages, and some activated CD4+ T cells (not resting). CD8a forms heterodimers with the CD8 beta chain (CD8b) on the surface of most thymocytes, while mature peripheral T lymphocytes express almost exclusively the CD8 alpha beta heterodimer. Intestinal intraepithelial lymphocytes express CD8a without CD8b. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for CD8 alpha Antibody (NBP1-42110). (Showing 1 - 5 of 5 FAQ).

  1. I'm looking for a good anti CD8  antibody working on immunofluorescence on murine tumoral tissue fixed in paraformaldehyde, cryoprotected with sucrose, frozen in OCT medium and sectioned with a cryostat.
    • I would recommend our CD8 alpha antibody NBP1-49045, it is guaranteed to work in IHC-P and and IHC-FR.   
  2. How do I know if this will actually work in IHC?
    • This CD8 alpha antibody has been validated in IHC and has been cited in a paper where it was used in IHC.
  3. Will this CD8 alpha antibody work for rabbit in IHC-FR?
    • Unfortunately this CD8 alpha antibody has not yet been validated in rabbit and there no sequence info in uniprot yet so we can't speculate based on homology. You may want to take advantage of our innovator's reward program which will allow you to test in rabbit without any financial risk. 
  4. Does this CD8 alpha antibody detect both forms of CD8?
    • Both the alpha - beta heterodimer as well as the alpha - alpha homodimer are detected by the (53-6.7) CD8 alpha antibody.
  5. Would you recommend your CD8 alpha antibody as a T-Cell marker? 
    • Yes this CD8 alpha anitbody has been cited in a study where it was used in FACS to separate out CD8+ T-cells. Please see the references tab PMID: 1608966. It is important to note that our CD8 alpha antibody may also stain mature dendritic cells and NK cells.

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CD8 alpha - Marker for cytotoxic T lymphocytes
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Gene Symbol CD8A

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