CD8 alpha Antibody (CT-8) [FITC]

Images

 
Flow Cytometry: CD8 alpha Antibody (CT-8) [FITC] [NBP1-28271] - Analysis of avian blood lymphocytes- image courtesy of customer.
Flow Cytometry: CD8 alpha Antibody (CT-8) [FITC] [NBP1-28271] - Analysis of peripheral blood mononuclear cells by multiple staining.

Product Details

Summary
Reactivity Av, Ch, TrSpecies Glossary
Applications Flow, IHC, IHC-Fr, IHC-P, IP, ICC
Clone
CT-8
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

CD8 alpha Antibody (CT-8) [FITC] Summary

Immunogen
Chicken thymocytes and Ig-negative blood leukocytes
Specificity
alpha chain (34-kDa) of chicken CD8
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD8A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry <= 1 ug/10^6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Immunocytochemistry
Reviewed Applications
Read 1 Review rated 5
using
NBP1-28271 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for CD8 alpha Antibody (CT-8) [FITC]

  • CD8 antigen, alpha polypeptide (p32)
  • CD8
  • CD8a antigen
  • CD8a molecule
  • Leu2 T-lymphocyte antigen
  • Leu2
  • MAL
  • OKT8 T-cell antigen
  • p32
  • RPA-T8 antibody flow
  • RPA-T8 Antibody
  • RPA-T8 CD8
  • RPA-T8 Clone
  • RPA-T8 Flow
  • T cell co-receptor
  • T8 T-cell antigen
  • T-cell antigen Leu2
  • T-cell surface glycoprotein CD8 alpha chain
  • T-lymphocyte differentiation antigen T8/Leu-2

Background

In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two alpha chains, and (ii) a heterodimer of an alpha chain and a beta chain. (1) While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8alpha- and CD8beta-chains, a relatively large proportion of the CD8+ TCR- gamma delta cells in the spleens of embryos and young chicks express only the alpha-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR-gamma delta CD8 alpha beta cells in the chicken that is not found in rodents. (1,3) Chicken CD8 is expressed on approximately 80% of thymocytes, 15% of blood mononuclear cells and 50% of spleen cells, but <1% of cells in the bursa and bone marrow. (1,2) MAb CT-8 recognizes the CD8alpha chain.1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP2-24867
Species: Hu, Mu
Applications: Flow, Func, In vitro, In vivo, CyTOF-ready, Flow-CS
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
H00003669-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
NB100-77417
Species: Mu
Applications: WB, Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vivo, CyTOF-ready
NB120-6405
Species: Rt
Applications: B/N, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
MAB342
Species: Hu
Applications: WB, AgAct
NBP1-92684
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ICC/IF
NBP1-26666
Species: Mu
Applications: B/N, Flow, IP, In vitro
NBP2-15184
Species: Hu, Rt, Ze
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NBP2-25196
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, In vitro, CyTOF-ready
NBP1-28271
Species: Av, Ch, Tr
Applications: Flow, IHC, IHC-Fr, IHC-P, IP, ICC

Publications for CD8 alpha Antibody (NBP1-28271) (0)

There are no publications for CD8 alpha Antibody (NBP1-28271).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for CD8 alpha Antibody (NBP1-28271) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Avian.
We have 1 review tested in 1 application: Flow.

Reviews using NBP1-28271:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Avian
(1)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry CD8 alpha NBP1-28271
Enlarge
reviewed by:
Revathi Shanmugasundaram
Flow Avian 02/28/2017
View

Summary

ApplicationFlow Cytometry
Sample TestedBlood lymphocytes
SpeciesAvian

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD8 alpha Antibody (NBP1-28271). (Showing 1 - 5 of 5 FAQ).

  1. I'm looking for a good anti CD8  antibody working on immunofluorescence on murine tumoral tissue fixed in paraformaldehyde, cryoprotected with sucrose, frozen in OCT medium and sectioned with a cryostat.
    • I would recommend our CD8 alpha antibody NBP1-49045, it is guaranteed to work in IHC-P and and IHC-FR.   
  2. How do I know if this will actually work in IHC?
    • This CD8 alpha antibody has been validated in IHC and has been cited in a paper where it was used in IHC. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3387923/
  3. Will this CD8 alpha antibody work for rabbit in IHC-FR?
    • Unfortunately this CD8 alpha antibody has not yet been validated in rabbit and there no sequence info in uniprot yet so we can't speculate based on homology. You may want to take advantage of our innovator's reward program which will allow you to test in rabbit without any financial risk. 
  4. Does this CD8 alpha antibody detect both forms of CD8?
    • Both the alpha - beta heterodimer as well as the alpha - alpha homodimer are detected by the (53-6.7) CD8 alpha antibody.
  5. Would you recommend your CD8 alpha antibody as a T-Cell marker? 
    • Yes this CD8 alpha anitbody has been cited in a study where it was used in FACS to separate out CD8+ T-cells. Please see the references tab PMID: 1608966. It is important to note that our CD8 alpha antibody may also stain mature dendritic cells and NK cells.

Secondary Antibodies

 

Isotype Controls

Additional CD8 alpha Products

Bioinformatics Tool for CD8 alpha Antibody (NBP1-28271)

Discover related pathways, diseases and genes to CD8 alpha Antibody (NBP1-28271). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CD8 alpha Antibody (NBP1-28271)

Discover more about diseases related to CD8 alpha Antibody (NBP1-28271).
 

Pathways for CD8 alpha Antibody (NBP1-28271)

View related products by pathway.

PTMs for CD8 alpha Antibody (NBP1-28271)

Learn more about PTMs related to CD8 alpha Antibody (NBP1-28271).
 

Research Areas for CD8 alpha Antibody (NBP1-28271)

Find related products by research area.

Blogs on CD8 alpha.

CD8 alpha - Marker for cytotoxic T lymphocytes
The T-cell co-receptor CD8 is a cell-surface glycoprotein that bridges the lipid bilayer by interacting externally with ligands, such as major histocompatibility complex (MHC) class I, as well as internally with signaling molecules such as tyrosine ki...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Revathi Shanmugasundaram
02/28/2017
Application: Flow
Species: Avian

Bioinformatics

Gene Symbol CD8A