CD8 alpha Antibody (76-2-11)

Images

 
Flow Cytometry: CD8 alpha Antibody (76-2-11) [NBP1-28237] - Porcine peripheral blood lymphocytes were stained with Mouse Anti-Porcine CD8 alpha-APC and Mouse Anti-Porcine CD4-FITC.
Flow Cytometry: CD8 alpha Antibody (76-2-11) [NBP1-28237] - Analysis using the FITC conjugate of NBP1-28237. CD8 alpha Antibody (76-2-11) [FITC] 1 ug/10^6 Peripheral blood mononuclear cells were isolated from ...read more

Product Details

Summary
Reactivity PoSpecies Glossary
Applications B/N, Flow, IHC-Fr, IHC-P, IP
Clone
76-2-11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

CD8 alpha Antibody (76-2-11) Summary

Immunogen
Fresh dd miniature swine thymocytes
Specificity
Porcine CD8a, Mr 32-34 kDa
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD8A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Blocking/Neutralizing 50-200 molar excess
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD8 alpha Antibody (76-2-11)

  • CD8 antigen, alpha polypeptide (p32)
  • CD8
  • CD8a antigen
  • CD8a molecule
  • Leu2 T-lymphocyte antigen
  • Leu2
  • MAL
  • OKT8 T-cell antigen
  • p32
  • RPA-T8 antibody flow
  • RPA-T8 Antibody
  • RPA-T8 CD8
  • RPA-T8 Clone
  • RPA-T8 Flow
  • T cell co-receptor
  • T8 T-cell antigen
  • T-cell antigen Leu2
  • T-cell surface glycoprotein CD8 alpha chain
  • T-lymphocyte differentiation antigen T8/Leu-2

Background

Porcine CD8, a type I transmembrane glycoprotein, is expressed as either a CD8 alpha alpha homodimer or a CD8 alpha beta heterodimer. It is found on most thymocytes, and on the 'suppressor/cytotoxic' subpopulation of peripheral T cells. CD8 functions primarily as a co-receptor with MHC Class I-restricted T-cell receptors in antigen recognition. MAb 76-2-11 reacts with the CD8 alpha chain. (1-3)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD8 alpha Antibody (NBP1-28237). (Showing 1 - 5 of 5 FAQ).

  1. I'm looking for a good anti CD8  antibody working on immunofluorescence on murine tumoral tissue fixed in paraformaldehyde, cryoprotected with sucrose, frozen in OCT medium and sectioned with a cryostat.
    • I would recommend our CD8 alpha antibody NBP1-49045, it is guaranteed to work in IHC-P and and IHC-FR.   
  2. How do I know if this will actually work in IHC?
    • This CD8 alpha antibody has been validated in IHC and has been cited in a paper where it was used in IHC. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3387923/
  3. Will this CD8 alpha antibody work for rabbit in IHC-FR?
    • Unfortunately this CD8 alpha antibody has not yet been validated in rabbit and there no sequence info in uniprot yet so we can't speculate based on homology. You may want to take advantage of our innovator's reward program which will allow you to test in rabbit without any financial risk. 
  4. Does this CD8 alpha antibody detect both forms of CD8?
    • Both the alpha - beta heterodimer as well as the alpha - alpha homodimer are detected by the (53-6.7) CD8 alpha antibody.
  5. Would you recommend your CD8 alpha antibody as a T-Cell marker? 
    • Yes this CD8 alpha anitbody has been cited in a study where it was used in FACS to separate out CD8+ T-cells. Please see the references tab PMID: 1608966. It is important to note that our CD8 alpha antibody may also stain mature dendritic cells and NK cells.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-28237AF405
Alexa Fluor 488 Labeled NBP1-28237AF488
Alexa Fluor 647 Labeled NBP1-28237AF647
Alexa Fluor 700 Labeled NBP1-28237AF700
Allophycocyanin Labeled NBP1-28237APC
DyLight 350 Labeled NBP1-28237UV
DyLight 405 Labeled NBP1-28237V
DyLight 488 Labeled NBP1-28237G
DyLight 680 Labeled NBP1-28237FR
DyLight 755 Labeled NBP1-28237IR
FITC Labeled NBP1-28238
PE Labeled NBP1-28237PE
PerCP Labeled NBP1-28237PCP

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CD8 alpha - Marker for cytotoxic T lymphocytes
The T-cell co-receptor CD8 is a cell-surface glycoprotein that bridges the lipid bilayer by interacting externally with ligands, such as major histocompatibility complex (MHC) class I, as well as internally with signaling molecules such as tyrosine ki...  Read full blog post.

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Bioinformatics

Gene Symbol CD8A