CD8 alpha Antibody (53-6.7)

Images

 
Immunocytochemistry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of immersion fixed splenocytes. Primary antibody was used at a dilution of 10 ug/ml and incubated for 3 hours at room temperature.
Immunohistochemistry-Frozen: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of CD8+ T cells in allogeneic skin grafted onto a mouse.
Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by multiple staining.
Immunocytochemistry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of bone marrow tissue by single and multiple staining.
Immunocytochemistry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of splenocytes. Primary antibody was used at a dilution of 10 ug/ml and incubated for 3 hours at room temperature.
Immunohistochemistry-Paraffin: CD8 alpha Antibody (53-6.7) [NBP1-49045] - CD8 alpha expression in mouse spleen tissue using anti-CD8 alpha antibody. Image from verified customer review.
Immunohistochemistry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of frozen spleen sections. Blocking: 1 hour with serum at 5% concentration and temperature of 25C.
Immunohistochemistry-Frozen: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of mosue spleen sections. CD8+ lymphocytes are marked by brown labeling of the cell surface.
Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - C57BL/6 mouse splenocytes were stained with biotinylated CD8 (clone 53-6.7) (filled histogram) or rat IgG2a, ? isotype control (open histogram), followed by ...read more
Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - C57BL/6 mouse splenocytes were stained with purified CD8 (clone 53-6.7) (filled histogram) or rat IgG2a, k isotype control (open histogram), followed by ...read more
Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of fixed murine splenocytes by multiple staining.
Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Staining of peripheral blood leukocytes.

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
Clone
53-6.7
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1 mg/ml

CD8 alpha Antibody (53-6.7) Summary

Description
This CD8 alpha antibody serves as an effective marker of cytotoxic T lymphocytes by binding to the CD8 co-receptor expressed on the cell surface of cytotoxic T cells, recognizing the topological domain of CD8 alpha. Because this CD8 antibody is made to the alpha chain it will recognize both the CD8 alpha - CD8 beta heterodimer, the most common form, as well as the CD8 alpha - CD8 alpha homodimer. The CD8A gene is also expressed in natural killer cells (NK cells), dendritic cells and cortical thymocytes making the CD8 alpha antibody a potential marker for these cells.
Immunogen
Mouse thymus or spleen.
Localization
Most thymocytes, T cell subset, some NK cells
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
CD8A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Cell depletion
  • CyTOF-ready
  • Inhibition of T Cell Function
Application Notes
Each lot of this CD8a antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is <0.25ug/10^6 cells in 100ul volume. It is recommended that the reagent be titrated for optimal performance for each application. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation, in vivo and in vitro cell depletion, inhibition of CD8 T cell proliferation, blocking of cytotoxicity, and immunohistochemical staining of both acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Immunocytochemistry/Immunofluorescence was reported in scientific literature. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24565643)
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-49045 in the following applications:

Publications
Read Publications using
NBP1-49045 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for CD8 alpha Antibody (53-6.7)

  • 53-6.7 CD8 Alpha
  • 53-6.7 CD8 Aplha
  • 53-6.7 CD8
  • 53-6.7 Clone
  • 53-6.7
  • CD8 Antibody
  • CD8 antigen, alpha polypeptide (p32)
  • CD8
  • CD8a antigen
  • CD8a molecule
  • Cytotoxic T cell marker
  • Cytotoxic T lymphocyte marker
  • Leu2 T-lymphocyte antigen
  • Leu2
  • MAL
  • OKT8 T-cell antigen
  • p32
  • RPA-T8 antibody flow
  • RPA-T8 Antibody
  • RPA-T8 CD8
  • RPA-T8 Clone
  • RPA-T8 Flow
  • T cell co-receptor
  • T cell marker
  • T8 T-cell antigen
  • T-cell antigen Leu2
  • T-cell surface glycoprotein CD8 alpha chain
  • T-lymphocyte differentiation antigen T8/Leu-2

Background

CD8, also known as MAL, p32, Leu2 and T8-T cell antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a 34kD protein that belongs to the immunoglobulin family and consists of disulfide-linked alpha and beta chains that form the alpha (CD8a)/beta (CD8b) heterodimer and alpha/alpha homodimer. The CD8 alpha/beta heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR alpha/beta T cells. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. Multiple transcript variants encoding different isoforms have been found for this gene. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Studies from Cambridge with the CD8 alpha antibody have shown 2 different types of tolerance (permanent or reversible) are induced in peripheral T-cells depending on the type of antigen they are exposed to(1). Associated studies with the same subjects used the CD8 alpha antibody to show that a simple monoclonal CD4 and CD8 monoclonal antibody therapy can establish transplantation tolerance(2). Another study in Takahashi's lab involving CD4 and CD8 used the CD8 alpha antibody in order to map signal molecules implicated in mediating the effects of interleukin 2 on T-cells(3). PMIDs: 1. 1702726 2. 1980112 3. 1608966

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD8 alpha Antibody (NBP1-49045)(9)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: FLOW, ICC/IF, IHC, IHC-P.


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FLOW
(1)
ICC/IF
(3)
IHC
(1)
IHC-P
(1)
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(6)
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Showing Publications 1 - 9 of 9.
Publications using NBP1-49045 Applications Species
Sierra RA, Trillo-Tinoco J, Mohamed E et al. Anti-Jagged immunotherapy inhibits MDSCs and overcomes tumor-induced tolerance Cancer Res. 2017 Sep 13 [PMID: 28904063] (ICC/IF, Mouse) ICC/IF Mouse
Cohen N, Shani O, Raz Y et al. Fibroblasts drive an immunosuppressive and growth-promoting microenvironment in breast cancer via secretion of Chitinase 3-like 1. Oncogene. Apr 3 2017 [PMID: 28368410] (ICC/IF, Mouse) ICC/IF Mouse
Li A, Wu Y, Tang F et al. In Vivo Magnetic Resonance Imaging of CD8+ T Lymphocytes Recruiting to Glioblastoma in Mice. Cancer Biother. Radiopharm. Nov 1 2016 [PMID: 27831762] (Mouse) Mouse
Fazio F, Zappulla C, Notartomaso S et al. Cinnabarinic acid, an endogenous agonist of type-4 metabotropic glutamate receptor, suppresses experimental autoimmune encephalomyelitis in mice. Neuropharmacology 2014 Mar 3 [PMID: 24565643] (IHC-P, Mouse) IHC-P Mouse
KheradmT, Wang S, Gibly R et al. Permanent protection of PLG scaffold transplanted allogeneic islet grafts in diabetic mice treated with ECDI-fixed donor splenocyte infusions Biomaterials 2011 Jul [PMID: 21458857] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Bankoti J, Burnett A, Navarro S et al. Effects of TCDD on the fate of naive dendritic cells. Toxicol Sci. 2010 Jun. [PMID: 20211938]
McLellan AD, Kapp M, Eggert A et al. Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression. Blood. [PMID: 11877283] (FLOW, Mouse) FLOW Mouse
Takahashi K, Nakata M, Tanaka T et al. CD4 and CD8 regulate interleukin 2 responses of T cells. Proc Natl Acad Sci U S A. 1992 Jun. [PMID: 1608966]
Ledbetter JA, Herzenberg LA. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979 [PMID: 398327]

Reviews for CD8 alpha Antibody (NBP1-49045) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.
We have 2 reviews tested in 1 application: IHC-P.

Reviews using NBP1-49045:
Filter by Applications
IHC-P
(2)
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Filter by Species
Mouse
(2)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin CD8 alpha NBP1-49045
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reviewed by:
Anonymous
IHC-P Mouse 10/23/2017
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse Head and neck squamous cell carcinoma (HNSCC)
SpeciesMouse
LotNBP1-49045

Comments

Commentssections were incubated with antibody for CD8 alpha (Novus, 1:200, for mouse HNSCC samples) at 4 °C for 12 h.
Immunohistochemistry-Paraffin CD8 alpha NBP1-49045
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reviewed by:
Naiche Adler
IHC-P Mouse 12/15/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Spleen
SpeciesMouse

Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for CD8 alpha Antibody (NBP1-49045). (Showing 1 - 5 of 5 FAQs).

  1. I'm looking for a good anti CD8  antibody working on immunofluorescence on murine tumoral tissue fixed in paraformaldehyde, cryoprotected with sucrose, frozen in OCT medium and sectioned with a cryostat.
    • I would recommend our CD8 alpha antibody NBP1-49045, it is guaranteed to work in IHC-P and and IHC-FR.   
  2. How do I know if this will actually work in IHC?
    • This CD8 alpha antibody has been validated in IHC and has been cited in a paper where it was used in IHC. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3387923/
  3. Will this CD8 alpha antibody work for rabbit in IHC-FR?
    • Unfortunately this CD8 alpha antibody has not yet been validated in rabbit and there no sequence info in uniprot yet so we can't speculate based on homology. You may want to take advantage of our innovator's reward program which will allow you to test in rabbit without any financial risk. 
  4. Does this CD8 alpha antibody detect both forms of CD8?
    • Both the alpha - beta heterodimer as well as the alpha - alpha homodimer are detected by the (53-6.7) CD8 alpha antibody.
  5. Would you recommend your CD8 alpha antibody as a T-Cell marker? 
    • Yes this CD8 alpha anitbody has been cited in a study where it was used in FACS to separate out CD8+ T-cells. Please see the references tab PMID: 1608966. It is important to note that our CD8 alpha antibody may also stain mature dendritic cells and NK cells.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-49045AF405
Alexa Fluor 488 Labeled NBP1-49045AF488
Alexa Fluor 647 Labeled NBP1-49045AF647
Alexa Fluor 700 Labeled NBP1-49045AF700
Allophycocyanin Labeled NBP1-49045APC
Biotin Labeled NBP1-49045B
DyLight 350 Labeled NBP1-49045UV
DyLight 405 Labeled NBP1-49045V
DyLight 488 Labeled NBP1-49045G
DyLight 550 Labeled NBP1-49045R
DyLight 650 Labeled NBP1-49045C
DyLight 680 Labeled NBP1-49045FR
DyLight 755 Labeled NBP1-49045IR
PE Labeled NBP1-49045PE
PerCP Labeled NBP1-49045PCP

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The T-cell co-receptor CD8 is a cell-surface glycoprotein that bridges the lipid bilayer by interacting externally with ligands, such as major histocompatibility complex (MHC) class I, as well as internally with signaling molecules such as tyrosine ki...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
10/23/2017
Application: IHC-P
Species: Mouse

Naiche Adler
12/15/2014
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol CD8A