CD8 alpha Antibody (12.C7) [DyLight 488]


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Product Details

Reactivity RbSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-P
Please see the vial label for concentration. If unlisted please contact technical services.

CD8 alpha Antibody (12.C7) [DyLight 488] Summary

Made to Rabbit CD8
Recognizes the rabbit CD8 cell surface antigen, expressed by a subset of T lymphocytes with cytotoxic/suppressor activity.
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C in the dark.
DyLight 488
DyLight 488 (A=493, E=518)
50mM Sodium Borate
0.05% Sodium Azide
Please see the vial label for concentration. If unlisted please contact technical services.
Protein G purified


Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for CD8 alpha Antibody (12.C7) [DyLight 488]

  • CD8 antigen, alpha polypeptide (p32)
  • CD8
  • CD8a antigen
  • CD8a molecule
  • Leu2 T-lymphocyte antigen
  • Leu2
  • MAL
  • OKT8 T-cell antigen
  • p32
  • RPA-T8 antibody flow
  • RPA-T8 Antibody
  • RPA-T8 CD8
  • RPA-T8 Clone
  • RPA-T8 Flow
  • T cell co-receptor
  • T8 T-cell antigen
  • T-cell antigen Leu2
  • T-cell surface glycoprotein CD8 alpha chain
  • T-lymphocyte differentiation antigen T8/Leu-2


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for CD8 alpha Antibody (NB100-64021G). (Showing 1 - 5 of 5 FAQ).

  1. I'm looking for a good anti CD8  antibody working on immunofluorescence on murine tumoral tissue fixed in paraformaldehyde, cryoprotected with sucrose, frozen in OCT medium and sectioned with a cryostat.
    • I would recommend our CD8 alpha antibody NBP1-49045, it is guaranteed to work in IHC-P and and IHC-FR.   
  2. How do I know if this will actually work in IHC?
    • This CD8 alpha antibody has been validated in IHC and has been cited in a paper where it was used in IHC.
  3. Will this CD8 alpha antibody work for rabbit in IHC-FR?
    • Unfortunately this CD8 alpha antibody has not yet been validated in rabbit and there no sequence info in uniprot yet so we can't speculate based on homology. You may want to take advantage of our innovator's reward program which will allow you to test in rabbit without any financial risk. 
  4. Does this CD8 alpha antibody detect both forms of CD8?
    • Both the alpha - beta heterodimer as well as the alpha - alpha homodimer are detected by the (53-6.7) CD8 alpha antibody.
  5. Would you recommend your CD8 alpha antibody as a T-Cell marker? 
    • Yes this CD8 alpha anitbody has been cited in a study where it was used in FACS to separate out CD8+ T-cells. Please see the references tab PMID: 1608966. It is important to note that our CD8 alpha antibody may also stain mature dendritic cells and NK cells.

Secondary Antibodies


Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB100-64021APC
DyLight 350 Labeled NB100-64021UV
DyLight 405 Labeled NB100-64021V
DyLight 405LS Labeled NB100-64021V3
DyLight 680 Labeled NB100-64021FR
DyLight 755 Labeled NB100-64021IR
PE Labeled NB100-64021PE
PerCP Labeled NB100-64021PCP

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Blogs on CD8 alpha.

CD8 alpha - Marker for cytotoxic T lymphocytes
The T-cell co-receptor CD8 is a cell-surface glycoprotein that bridges the lipid bilayer by interacting externally with ligands, such as major histocompatibility complex (MHC) class I, as well as internally with signaling molecules such as tyrosine ki...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol CD8A

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