CD8 alpha Antibody (12.C7)

Images

 
Flow Cytometry: CD8 alpha Antibody (12.C7) [NB100-64021] - Rabbit peripheral blood lymphocytes stained with Mouse anti Rabbit CD8 Alpha followed by Goat anti Mouse IgG (H/L):FITC

Product Details

Summary
Reactivity RbSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-P
Clone
12.C7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CD8 alpha Antibody (12.C7) Summary

Immunogen
Made to Rabbit CD8
Specificity
Recognizes the rabbit CD8 cell surface antigen, expressed by a subset of T lymphocytes with cytotoxic/suppressor activity.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD8A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100-1:200
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
ICC/IF, IHC reported in scientific literature (PMID: 22796166 )
Reviewed Applications
Read 1 Review rated 5
using
NB100-64021 in the following applications:

Publications
Read Publications using
NB100-64021 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD8 alpha Antibody (12.C7)

  • CD8 antigen, alpha polypeptide (p32)
  • CD8
  • CD8a antigen
  • CD8a molecule
  • Leu2 T-lymphocyte antigen
  • Leu2
  • MAL
  • OKT8 T-cell antigen
  • p32
  • RPA-T8 antibody flow
  • RPA-T8 Antibody
  • RPA-T8 CD8
  • RPA-T8 Clone
  • RPA-T8 Flow
  • T cell co-receptor
  • T8 T-cell antigen
  • T-cell antigen Leu2
  • T-cell surface glycoprotein CD8 alpha chain
  • T-lymphocyte differentiation antigen T8/Leu-2

Background

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD8 alpha Antibody (NB100-64021)(2)

We have publications tested in 1 confirmed species: Rabbit.

We have publications tested in 3 applications: ICC/IF, IHC, IHC-P.


Filter By Application
ICC/IF
(1)
IHC
(1)
IHC-P
(1)
All Applications
Filter By Species
Rabbit
(2)
All Species

Review for CD8 alpha Antibody (NB100-64021) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NB100-64021:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Other
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-P Other 12/27/2011
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedRabbit tissue
SpeciesOther
CommentsThis antibody was very useful for IHC-P expression.

Blocking

Blocking DetailsBlocking Buffer: 1% Goat Serum

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:10, Primary Ab Incubation Time: 24 hours, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: Dylight 488 goat anti-mouse IgG

Details

Detection NotesDetection Method: fluorescene micrograph

Comments

CommentsThis antibody was very useful for IHC-P expression.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for CD8 alpha Antibody (NB100-64021). (Showing 1 - 5 of 5 FAQs).

  1. I'm looking for a good anti CD8  antibody working on immunofluorescence on murine tumoral tissue fixed in paraformaldehyde, cryoprotected with sucrose, frozen in OCT medium and sectioned with a cryostat.
    • I would recommend our CD8 alpha antibody NBP1-49045, it is guaranteed to work in IHC-P and and IHC-FR.   
  2. How do I know if this will actually work in IHC?
    • This CD8 alpha antibody has been validated in IHC and has been cited in a paper where it was used in IHC. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3387923/
  3. Will this CD8 alpha antibody work for rabbit in IHC-FR?
    • Unfortunately this CD8 alpha antibody has not yet been validated in rabbit and there no sequence info in uniprot yet so we can't speculate based on homology. You may want to take advantage of our innovator's reward program which will allow you to test in rabbit without any financial risk. 
  4. Does this CD8 alpha antibody detect both forms of CD8?
    • Both the alpha - beta heterodimer as well as the alpha - alpha homodimer are detected by the (53-6.7) CD8 alpha antibody.
  5. Would you recommend your CD8 alpha antibody as a T-Cell marker? 
    • Yes this CD8 alpha anitbody has been cited in a study where it was used in FACS to separate out CD8+ T-cells. Please see the references tab PMID: 1608966. It is important to note that our CD8 alpha antibody may also stain mature dendritic cells and NK cells.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-64021AF405
Alexa Fluor 488 Labeled NB100-64021AF488
Alexa Fluor 647 Labeled NB100-64021AF647
Alexa Fluor 700 Labeled NB100-64021AF700
Allophycocyanin Labeled NB100-64021APC
DyLight 350 Labeled NB100-64021UV
DyLight 405 Labeled NB100-64021V
DyLight 488 Labeled NB100-64021G
DyLight 680 Labeled NB100-64021FR
DyLight 755 Labeled NB100-64021IR
PE Labeled NB100-64021PE
PerCP Labeled NB100-64021PCP

Additional Array Products

Bioinformatics Tool for CD8 alpha Antibody (NB100-64021)

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Research Areas for CD8 alpha Antibody (NB100-64021)

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Blogs on CD8 alpha.

CD8 alpha - Marker for cytotoxic T lymphocytes
The T-cell co-receptor CD8 is a cell-surface glycoprotein that bridges the lipid bilayer by interacting externally with ligands, such as major histocompatibility complex (MHC) class I, as well as internally with signaling molecules such as tyrosine ki...  Read full blog post.

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Recent Reviews

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Anonymous
12/27/2011
Application: IHC-P
Species: Other

Bioinformatics

Gene Symbol CD8A