CD79A Antibody

Images

 
Immunohistochemistry-Paraffin: CD79a Antibody [NB600-1058] - Staining of human tonsil. Image courtesy of product review by verified customer.
Immunohistochemistry-Paraffin: CD79a Antibody [NB600-1058] - IHC analysis of CD79a formalin-fixed paraffin embedded human tonsil using NB600-1058.

Product Details

Summary
Reactivity Hu, Po, Bv, Ch, Eq, GP, Pm, RbSpecies Glossary
Applications IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CD79A Antibody Summary

Immunogen
A synthetic peptide derived from the N-terminal region of human CD79a protein.
Localization
Cell membrane.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD79A
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Application Notes
Immunohistochemistry paraffin For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval using 10mM citrate buffer pH 6.0 before commencing with the IHC staining protocol.
Positive Control
CD79A Lysate (NBL1-08969)
Reviewed Applications
Read 1 Review rated 4
using
NB600-1058 in the following applications:

Publications
Read Publications using
NB600-1058 in the following applications:

Reactivity Notes

May react with Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
Sodium Azide
Purity
IgG purified

Alternate Names for CD79A Antibody

  • CD79A antigen (immunoglobulin-associated alpha)
  • CD79a antigen
  • CD79a molecule, immunoglobulin-associated alpha
  • CD79A
  • IGAB-cell antigen receptor complex-associated protein alpha chain
  • ig-alpha
  • MB-1 membrane glycoprotein
  • MB1
  • MB-1
  • Membrane-bound immunoglobulin-associated protein
  • Surface IgM-associated protein

Background

CD79a is a disulphide-linked heterodimer, consisting of CD79a / mb-1 and CD79b / B29 and is non-covalently associated with membrane-bound immunoglobulins on B cells to constitute the B cell antigen receptor. CD79a first appears at pre B cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas and in some myelomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD79A Antibody (NB600-1058)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC, IHC-P.


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IHC
(1)
IHC-P
(1)
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Human
(2)
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Review for CD79A Antibody (NB600-1058) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IHC-P.

Reviews using NB600-1058:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin CD79A NB600-1058
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reviewed by:
Xiaoyan Huang
IHC-P Human 02/13/2013
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman tonsil
SpeciesHuman
LotT350

Blocking

Blocking Detailsprotein blocking buffer(Dako), 30 to 60 minutes at RT

Primary Anitbody

Dilution Ratio1:10 to 1:50, overnight at 4 degree.

Secondary Antibody

Secondary DescriptionPolyclonal Goat Anti- Rabbit Immunoglobulins (Dako)
Secondary Concentration1:200

Details

Detection Notesliquid DAB+substrate chromogen system(Dako), 1-15 minutes
Fixation Details10% formalin fixed overnight, 10nM citrate buffer, ph6.1 (Dako, commercial ), at 95~99 °C for 30 min.
Wash DescriptionPBST, 5 minutes, 3 times

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD79A Antibody (NB600-1058). (Showing 1 - 2 of 2 FAQs).

  1. I have recently purchase a CD79a antibody NB600-1058 and was wondering if you could supply me with a concentration for this antibody.
    • Thank you for your inquiry. The total IgG concentration for NB600-1058 Lot T350 is 45ug/ml.
  2. What is the concentration of sodium azide for cat#NB600-1058?
    • NB600-1058 contains less than 0.1% sodium azide.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for CD79A Antibody (NB600-1058)

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Research Areas for CD79A Antibody (NB600-1058)

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Blogs on CD79A.

CD79b - A Signal Transduction Component of the B-cell Receptor
The B-cell antigen receptor (BCR) is a complex multimeric aggregate that includes the following key noncovalently-bound components: antigen-specific surface immunoglobulin (Ig), CD79a (Ig-alpha), and CD79b (Ig-beta). BCR signaling is a pivotal pathway...  Read full blog post.

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Recent Reviews

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Xiaoyan Huang
02/13/2013
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol CD79A
Entrez
Uniprot