CD47 Antibody

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Western Blot: CD47 Antibody [NBP2-42506] - Analysis of Human Hippocampus (A) and HeLa (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-42506
    • Availability
      Product Discontinued

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CD47 Antibody Summary

Immunogen
Peptide with sequence C-SNQKTIQPPRK corresponding to internal region (near C-Terminus) according to NP_001768.1, NP_942088.1.
Specificity
This antibody is expected to recognize both reported isoforms (NP_001768.1; NP_942088.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CD47
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 50 kDa band observed in human hippocampus lysates and 60 kDa band in lysates of HeLa and Jurkat (calculated MW of 35.2 kDa band according to NP_001768.1). The observed molecular weights correspond to glycosylated forms.
Control
CD47 Lysate (NBP2-65671)
Publications
Read Publication using NBP2-42506.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CD47 Antibody

  • antigen identified by monoclonal 1D8
  • Antigenic surface determinant protein OA3
  • CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
  • CD47 antigen
  • CD47 glycoprotein
  • CD47 molecule
  • CD47
  • IAP
  • IAPintegrin associated protein
  • Integrin-associated protein
  • leukocyte surface antigen CD47
  • MER6integrin-associated signal transducer
  • OA3
  • Protein MER6
  • Rh-related antigen

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD47 Antibody (NBP2-42506)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

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Bioinformatics

Gene Symbol CD47
Entrez