CD46 Antibody (177)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-60148
    • Availability
      Product Discontinued

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CD46 Antibody (177) Summary

Immunogen
Purified MCP
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD46
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Functional
  • Immunoassay
  • Immunoprecipitation 1:10-1:500
Application Notes
This antibody works in Flow Cytometry, Functional, Immunoassay, Immunoprecipitation, Western Blot. The typical starting working dilution is 1:10. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of CD46 to be inactivated.
Publications
Read Publications using NBP1-60148.

Reactivity Notes

This product is reactive in Human

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.2 um filtered solution in PBS and 0.1% BSA
Preservative
No Preservative
Purity
Protein A or G purified

Notes

Positive Control: CD46 is expressed on every cell and tissue Negative control: Erythrocytes

Alternate Names for CD46 Antibody (177)

  • AHUS2
  • CD46 antigen
  • CD46 molecule, complement regulatory protein
  • CD46
  • MCP
  • membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen)
  • MIC10
  • TLXcomplement regulatory protein
  • trophoblast leucocyte common antigen
  • Trophoblast leukocyte common antigen
  • trophoblast-lymphocyte cross-reactive antigen

Background

The monoclonal antibody M177 recognizes CD46, also designated membrane cofactor protein (MCP). CD46 is a 45-70 kDa protein with genetic and tissue-specific heterogeneity. It is expressed on every cell and tissue, with the exception of erythrocytes. CD46 serves to inhibit complement activation on host tissue. It performs this function by serving as a cofactor which binds to C3b and C4b. This binding is permitted by factor I, a serine protease of plasma, to degrade C3b and C4b and serves to protect the host cell against autologous attack. It also serves as a receptor for measles virus. Four isoforms of CD46 predominate and arise by alternative splicing of a single CD46 gene. CD46 cDNA encodes a signal sequence followed by four complement control protein domains (also called short consensus repeats (SCR)). The monoclonal antibody M177 reacts with the SCR2 domain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD46 Antibody (NBP1-60148)(7)


Showing Publications 1 - 7 of 7.
Publications using NBP1-60148 Applications Species
Matsui H, Sakurai F, Katayama K, Kurachi S, Tashiro K, Sugio K, Kawabata K, Mizuguchi H. Enhanced transduction efficiency of fiber-substituted adenovirus vectors by the incorporation of RGD peptides in two distinct regions of the adenovirus serotype 35 fiber knob. Source Department of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan. Virus Res. 155(1):48-54. 2011 Jan. [PMID: 20801174]
Kato S, Ohgimoto S, Sharma LB, Kurazono S, Ayata M, Komase K, Takeda M, Takeuchi K, Ihara T, Ogura H. Reduced ability of hemagglutinin of the CAM-70 measles virus vaccine strain to use receptors CD46 and SLAM. Source Department of Virology, Osaka City University Medical School, Osaka 545-8585, Japan. Vaccine. 27(29):3838-48. 2009 Jun 12. [PMID: 19490984]
Lore K, Adams WC, Havenga MJ, Precopio ML, Holterman L, Goudsmit J, Koup RA. Myeloid and plasmacytoid dendritic cells are susceptible to recombinant adenovirus vectors and stimulate polyfunctional memory T cell responses. Source Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA. karin.lore@ki.se. J Immunol. 179(3):1721-9. 2007 Aug 1. [PMID: 17641038]
Kurita-Taniguchi M, Hazeki K, Murabayashi N, Fukui A, Tsuji S, Matsumoto M, Toyoshima K, Seya T. Molecular assembly of CD46 with CD9, alpha3-beta1 integrin and protein tyrosine phosphatase SHP-1 in human macrophages through differentiation by GM-CSF. Source Department of Immunology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Higashinari-ku, Osaka, Japan. Mol Immunol. 38(9):689-700. 2002 Feb. [PMID: 11858824]
Kurita-Taniguchi M, Fukui A, Hazeki K, Hirano A, Tsuji S, Matsumoto M, Watanabe M, Ueda S, Seya T. Functional modulation of human macrophages through CD46 (measles virus receptor): production of IL-12 p40 and nitric oxide in association with recruitment of protein-tyrosine phosphatase SHP-1 to CD46. Source Department of Immunology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Higashinari-ku, Osaka, Japan. J Immunol. 165(9):5143-52. 2000 Nov 1. [PMID: 11046046]
Iwata K, Seya T, Yanagi Y, Pesando JM, Johnson PM, Okabe M, Ueda S, Ariga H, Nagasawa S. Diversity of sites for measles virus binding and for inactivation of complement C3b and C4b on membrane cofactor protein CD46. Source Division of Hygienic Chemistry, Hokkaido University, Sapporo, Japan. J Biol Chem. 270(25):15148-52. 1995 Jun 23. [PMID: 7541036]
Seya T, Hara T, Matsumoto M, Akedo H. Quantitative analysis of membrane cofactor protein (MCP) of complement. High expression of MCP on human leukemia cell lines, which is down-regulated during cell differentiation. Source Department of Immunology, Center for Adult Diseases, Osaka, Japan. J Immunol. 145(1):238-45. 1990 Jul 1. [PMID: 1694203]

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Bioinformatics

Gene Symbol CD46
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