CD45RO Antibody (UCHL1) [Alexa Fluor (R) 647]



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CD45RO Antibody (UCHL1) [Alexa Fluor (R) 647] Summary

IL-2 dependent T cell line, CA1.
Cell membrane
This antibody reacts with a 180 kD glycoprotein of CD45 family, occurring on most thymocytes and activated T cells but only on the portion of the resting T cells. It reacts with most thymocytes, a subpopulation of resting cells within both the CD4 and CD8 subsets, and mature activated T cells.
IgG2a Kappa
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C in the dark.
Alexa Fluor (R) 647
Alexa Fluor (R) 647
50mM Sodium Borate
0.05% Sodium Azide
Protein A or G purified


Application Notes
This antibody may be diluted to a titer of 1:25-1:50 in an ABC method.
We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilution should be determined by the user.
Overfixation in formalin should be avoided.
Enzymatic predigestion can cause false positive staining of non T cell tumors.

Reactivity Notes



This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for CD45RO Antibody (NB600-829AF647). (Showing 1 - 1 of 1 FAQ).

  1. I cannot find any anti-mouse CD45RO for FACS. Have any of your anti-Human CD45RO been tested on mouse T cells?
    • Unfortunately, none of our CD45RO antibodies for Flow Cytometry have been validated on mouse. If you would be interested in testing the antibody on mouse, we would recommend Our Innovators Reward Program. Under the terms and conditions of this program we would be happy to provide you with a credit of equal or lesser value, in exchange for your online review including an image of either positive or negative staining for testing in a new species and/or application. Please contact us at with any questions regarding this program.

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CD45RO NB600-829AF647

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