CD45.2 Antibody (104) [Biotin]

Images

 
Flow Cytometry: CD45.2 Antibody (104) [Biotin] [NBP1-43641] - Surface staining of BALB/c mouse splenocytes with Anti-Mouse CD45.2 PE (left), and APC-Cy7 (right). Appropriate isotype controls were used (open histogram). ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-43641
    • Availability
      Product Discontinued

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CD45.2 Antibody (104) [Biotin] Summary

Immunogen
B10.S mouse thymocytes and splenocytes
Specificity
CD45.2 (104)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PTPRC
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.25 microgram per one million cells in 100 microliter volume. Immunohistochemistry-frozen sections. It is recommended that the reagent be titrated for optimal performance for each application.
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Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD45.2 Antibody (104) [Biotin]

  • B220
  • CD 45
  • cd45 antigen
  • CD45.2
  • CD45R
  • GP 180
  • GP180
  • Human homolog of severe combined immunodeficiency due to PTPRC deficiency
  • L CA
  • LCA
  • Leukocyte common antigen
  • LY 5
  • Ly5
  • Ly5.2
  • Ly-5.2
  • Protein tyrosine phosphatase receptor type c polypeptide
  • Protein tyrosine phosphatase receptor type C
  • PTPRC
  • RT7.2
  • SCID due to PTPRC deficiency
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • T200

Background

The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains. The strains that express CD45.2 include the most commonly used mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. CD45.2 is expressed by all leukocytes in these strains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Additional CD45.2 Products

CD45.2 NBP1-43641

Bioinformatics Tool for CD45.2 Antibody (NBP1-43641)

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Bioinformatics

Gene Symbol PTPRC
Entrez