CD45 Antibody (2B11+ PD7/26/16)



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CD45 Antibody (2B11 PD7/26/16) Summary

2BII - T cell lymphoma cells, PD7/26/16 - human peripheral lymphocytes
NB100-65096 is a mixture of two clones recognizing the human CD45 cell surface antigen, also known as the leucocyte common antigen. This reagent is useful for the differentiation of tissues of haemic origin from other tissues or tumours. Cells of lymphoid lineage show the most intense staining.
Tissue culture supernatant
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 1% Bovine Serum Albumin
0.09% Sodium Azide
This product is unpurified. The exact concentration of antibody is not quantifiable.
Tissue culture supernatant


Application Notes
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

Reactivity Notes

Reacts with Human. Cross reacts with Cynomolgus Monkey.


CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognizing a common epitope on all of the isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for CD45 Antibody (NB100-65096). (Showing 1 - 2 of 2 FAQ).

  1. We are interested in CD45 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • I would recommend one of these CD45 antibodies to you, as these have all been validated for use with dog/canine samples.
  2. We would like to order a CD45 antibody to stain immune cells that were isolated from a ligated sciatic nerve of mice with double immunofluorescence (using PFA-fixed tissue on slides). Which of the following rat monoclonal CD45 antibodies would you recommend: 30-F11, IBL-3/16 or 5C16?
    • I would recommend clone 30-F11. As clone IBL-3/16 has not yet been validated for IHC-P application, we would not be able to guarantee its workability on PFA-fixed tissue. Although both other clones i.e. 30-F11 and 5C16, would be good for your samples, CD45 30-F11 # NB100-77417 is a well known clone that offers more flexibility around protocol because of availability of its conjugated forms. It would be advantageous to use a conjugated primary as you are planning for double-immunostaining procedure.

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CD45 - Much more than just a housekeeping protein
CD45, also known as T200 or the Leukocyte Common Antigen (LCA), is encoded by the Protein Tyrosine Phosphatase Receptor type C (PTPRC) gene. The protein is expressed exclusively on cells of the haematopoietic system, and is one of the most abundant le...  Read full blog post.

CD45 Isoforms: Hematopoietic Differentiation, Cancer and Alzheimer's
CD45, also known as protein tyrosine phosphatase, receptor type, C (PTPRC), was originally known as common leukocyte antigen and is a signal transducer involved in many physiological processes such as growth and differentiation, cancer transformation,...  Read full blog post.

Vimentin Antibodies in Rheumatoid Arthritis & Cataracts Research
Vimentin is a 57kDa type III intermediate filament (IF) protein that is the major cytoskeletal component of mesenchymal cells and the first to be expressed during cell differentiation. It plays a significant role in supporting and anchoring the positi...  Read full blog post.

Antibodies In The Differential Diagnosis Of Undifferentiated Tumors
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Gene Symbol PTPRC

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