CD44, variant 4 Antibody (VFF-11)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr, IHC-P
Clone
VFF-11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

CD44, variant 4 Antibody (VFF-11) Summary

Immunogen
Glutathione S Transferase (GST) fusion protein corresponding to the variable domains (v3 to v10) of human CD44.
Marker
Cell Membrane Marker
Specificity
Specifically recognizes an epitope encoded by exon v4 on the variant portion of human CD44.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD44
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:50-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections; sodium citrate buffer pH 6.0 is recommended for this purpose. For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul.
Publications
Read Publication using NB100-64995.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
0.01% Thimerosal
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD44, variant 4 Antibody (VFF-11)

  • CD44 antigen
  • CD44 molecule (Indian blood group)
  • CD44R
  • CDw44
  • cell surface glycoprotein CD44
  • chondroitin sulfate proteoglycan 8
  • CSPG8
  • ECMR-III
  • epican
  • Extracellular matrix receptor III
  • GP90 lymphocyte homing/adhesion receptor
  • HCELL
  • hematopoietic cell E- and L-selectin ligand
  • Heparan sulfate proteoglycan
  • Hermes antigen
  • homing function and Indian blood group system
  • HUTCH-I
  • Hyaluronate receptor
  • IN
  • LHR
  • MC56
  • MDU2CD44 antigen (homing function and Indian blood group system)
  • MDU3CDW44
  • MIC4MGC10468
  • Pgp1
  • PGP-1
  • PGP-I
  • Phagocytic glycoprotein 1
  • Phagocytic glycoprotein I

Background

CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD44, variant 4 Antibody (NB100-64995)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-64995AF405
Alexa Fluor 488 Labeled NB100-64995AF488
Alexa Fluor 647 Labeled NB100-64995AF647
Alexa Fluor 700 Labeled NB100-64995AF700
Allophycocyanin Labeled NB100-64995APC
DyLight 350 Labeled NB100-64995UV
DyLight 405 Labeled NB100-64995V
DyLight 488 Labeled NB100-64995G
DyLight 680 Labeled NB100-64995FR
DyLight 755 Labeled NB100-64995IR
PE Labeled NB100-64995PE
PerCP Labeled NB100-64995PCP

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CD44, variant 4 NB100-64995

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Bioinformatics

Gene Symbol CD44