Human CD44 ELISA Kit (Colorimetric)


ELISA: CD44 ELISA Kit [NBP1-86819] - Standard curve
ELISA: Human CD44 ELISA Kit (Colorimetric) [NBP1-86819] - The CD44std ELISA detects all circulating CD44 isoforms comprising the standard protein sequences. Standard protein sequences in black, variant exons, open more

Product Details

Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), amniotic fluid, urine
Standard Curve Range
0.13 - 4.0 ng/mL
0.02 ng/mL

Order Details

Human CD44 ELISA Kit (Colorimetric) Summary

This antibody targets the soluble protein. The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human CD44std positive serum. There was no crossreactivity detected, namely not with TNF alpha, TNF beta, TNF-R, IFN alpha 2c, IFN gamma, IL-8, Annexin, ELAM-1, L-selectin, ICAM-1 and HER-2.
Standard Curve Range
0.13 - 4.0 ng/mL
0.02 ng/mL
Assay Type
Sandwich ELISA
Spike Recovery
The amount of endogenous human CD44std in unspiked serum was subtracted from the spike values. The recovery ranged from 76% to 101% with an overall mean recovery of 89%.
Sample Volume
20 uL
Kit Type
ELISA Kit (Colorimetric)


Application Notes
This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Reactivity Notes


Packaging, Storage & Formulations

Storage of components varies. See protocol for specific instructions.

Kit Components

  1. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human C1q

Alternate Names for Human CD44 ELISA Kit (Colorimetric)

  • CD44 antigen
  • CD44 molecule (Indian blood group)
  • CD44
  • CD44R
  • CDw44
  • cell surface glycoprotein CD44
  • chondroitin sulfate proteoglycan 8
  • CSPG8
  • epican
  • Extracellular matrix receptor III
  • GP90 lymphocyte homing/adhesion receptor
  • HCAM
  • hematopoietic cell E- and L-selectin ligand
  • Heparan sulfate proteoglycan
  • Hermes antigen
  • homing function and Indian blood group system
  • Hyaluronate receptor
  • IN
  • LHR
  • MC56
  • MDU2
  • MDU2CD44 antigen (homing function and Indian blood group system)
  • MDU3
  • MDU3CDW44
  • MIC4
  • MIC4MGC10468
  • Pgp1
  • PGP-1
  • PGP-I
  • Phagocytic glycoprotein 1
  • Phagocytic glycoprotein I


CD44 (Pgp-1; Ly-24; ECMR III; F10-44-2; H-CAM; HUTCH-I; In(Lu)- related p80; Hermes antigen; hyaluronan receptor) is a polymorphic glycoprotein with apparent molecular weights ranging from 85 kDa to 250 kDa. This cell membrane associated molecule has a cytoplasmic tail (mediates the interaction with the cytoskeleton), a short hydrophobic transmembrane region and an NH2-terminal extracellular (binds to hyaluronate) domain. CD44 isoforms participate in a wide variety of cell-cell or cell-matrix interactions including lymphocyte homing, establishment of B and T cell immune responses, tumor metastases formation and inflammation. Three isoform categories of the CD44 molecule have been identified: - an 80-90 kDa isoform, the so-called standard form named CD44std, which is widely distributed on several hematopoietic and nonhemato- poietic cells including all subsets of leukocytes, monocytes, erythrocytes, many types of epithelium, mesenchymal elements like fibroblasts, smooth muscle cells and glial cells of the central nervous system, - a medium size category of 110-160 kDa, which is weakly expressed on epithelial cells and highly expressed in some carcinomas, and - a category that includes very large isoforms of 250 kDa covalently modified by the addition of chondroitin sulfate. These bigger isoforms of CD44 arise by alternative splicing of one or more "variant" exons (v2-v10) into the extracellular part of the 90kDa constant form molecule. Compared to the standard CD44, all larger isoforms are expressed in a much more restricted fashion, only in a few normal tissues or on the surface of certain tumor cells. Some splice variants of CD44 play important and distinct roles in tumor metastasis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Gene Symbol CD44