Human CD44 ELISA Kit (Colorimetric)

Images

 
ELISA: CD44 ELISA Kit [NBP1-86819] - Standard curve
ELISA: Human CD44 ELISA Kit (Colorimetric) [NBP1-86819] - The CD44std ELISA detects all circulating CD44 isoforms comprising the standard protein sequences. Standard protein sequences in black, variant exons, open ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), amniotic fluid, urine
Standard Curve Range
0.13 - 4.0 ng/mL
Sensitivity
0.02 ng/mL

Order Details

Human CD44 ELISA Kit (Colorimetric) Summary

Specificity
This antibody targets the soluble protein. The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human CD44std positive serum. There was no crossreactivity detected, namely not with TNF alpha, TNF beta, TNF-R, IFN alpha 2c, IFN gamma, IL-8, Annexin, ELAM-1, L-selectin, ICAM-1 and HER-2.
Standard Curve Range
0.13 - 4.0 ng/mL
Sensitivity
0.02 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
4.1%
Intra-Assay
4.8%
Spike Recovery
The amount of endogenous human CD44std in unspiked serum was subtracted from the spike values. The recovery ranged from 76% to 101% with an overall mean recovery of 89%.
Sample Volume
20 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CD44

Applications/Dilutions

Application Notes
This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human C1q

Alternate Names for Human CD44 ELISA Kit (Colorimetric)

  • CD44 antigen
  • CD44 molecule (Indian blood group)
  • CD44
  • CD44R
  • CDw44
  • cell surface glycoprotein CD44
  • chondroitin sulfate proteoglycan 8
  • CSPG8
  • ECMR-III
  • epican
  • Extracellular matrix receptor III
  • GP90 lymphocyte homing/adhesion receptor
  • HCAM
  • HCELL
  • hematopoietic cell E- and L-selectin ligand
  • Heparan sulfate proteoglycan
  • Hermes antigen
  • homing function and Indian blood group system
  • HUTCH-I
  • Hyaluronate receptor
  • IN
  • LHR
  • MC56
  • MDU2
  • MDU2CD44 antigen (homing function and Indian blood group system)
  • MDU3
  • MDU3CDW44
  • MIC4
  • MIC4MGC10468
  • MUTCH-I
  • Pgp1
  • PGP-1
  • PGP-I
  • Phagocytic glycoprotein 1
  • Phagocytic glycoprotein I

Background

CD44 (Pgp-1; Ly-24; ECMR III; F10-44-2; H-CAM; HUTCH-I; In(Lu)- related p80; Hermes antigen; hyaluronan receptor) is a polymorphic glycoprotein with apparent molecular weights ranging from 85 kDa to 250 kDa. This cell membrane associated molecule has a cytoplasmic tail (mediates the interaction with the cytoskeleton), a short hydrophobic transmembrane region and an NH2-terminal extracellular (binds to hyaluronate) domain. CD44 isoforms participate in a wide variety of cell-cell or cell-matrix interactions including lymphocyte homing, establishment of B and T cell immune responses, tumor metastases formation and inflammation. Three isoform categories of the CD44 molecule have been identified: - an 80-90 kDa isoform, the so-called standard form named CD44std, which is widely distributed on several hematopoietic and nonhemato- poietic cells including all subsets of leukocytes, monocytes, erythrocytes, many types of epithelium, mesenchymal elements like fibroblasts, smooth muscle cells and glial cells of the central nervous system, - a medium size category of 110-160 kDa, which is weakly expressed on epithelial cells and highly expressed in some carcinomas, and - a category that includes very large isoforms of 250 kDa covalently modified by the addition of chondroitin sulfate. These bigger isoforms of CD44 arise by alternative splicing of one or more "variant" exons (v2-v10) into the extracellular part of the 90kDa constant form molecule. Compared to the standard CD44, all larger isoforms are expressed in a much more restricted fashion, only in a few normal tissues or on the surface of certain tumor cells. Some splice variants of CD44 play important and distinct roles in tumor metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

7268-CT
NBP1-84550
Species: Hu
Applications: WB, IHC, IHC-P
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
796-IC
Species: Mu
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-77417
Species: Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vivo, CyTOF-ready
AF728
Species: Hu
Applications: WB, AdBlk
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
NBP1-91258
Species: Hu, Mu, Bv, Ca, Fe
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-36561
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC-Fr, IP, B/N
NB600-1071
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
AF1730
Species: Hu
Applications: Flow, AdBlk, CyTOF-ready, ICC
MAB5248
Species: Hu
Applications: IHC
NB500-328
Species: Hu
Applications: Flow, IP, CyTOF-ready
AF6585
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready
AF808
Species: Mu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
7270-IL/CF
Species: Hu
AF2364
Species: Hu
Applications: WB, IHC

Publications for CD44 ELISA Kit (NBP1-86819) (0)

There are no publications for CD44 ELISA Kit (NBP1-86819).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CD44 ELISA Kit (NBP1-86819) (0)

There are no reviews for CD44 ELISA Kit (NBP1-86819). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD44 ELISA Kit (NBP1-86819) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for CD44 ELISA Kit (NBP1-86819)

Discover related pathways, diseases and genes to CD44 ELISA Kit (NBP1-86819). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CD44 ELISA Kit (NBP1-86819)

Discover more about diseases related to CD44 ELISA Kit (NBP1-86819).
 

Pathways for CD44 ELISA Kit (NBP1-86819)

View related products by pathway.

PTMs for CD44 ELISA Kit (NBP1-86819)

Learn more about PTMs related to CD44 ELISA Kit (NBP1-86819).
 

Research Areas for CD44 ELISA Kit (NBP1-86819)

Find related products by research area.

Blogs on CD44.

The Osteopontin Antibody and Hepatic Research
Antibody suppliers, such as us at Novus Biologicals, supply a wide range of cell marker products, among them the Osteopontin antibody. Recently, the osteopontin antibody has proven useful in hepatic cancer research.Osteopontin (OPN) is mainly expres...  Read full blog post.

CD Antibodies Uncover Markers for Rare Breast Cancer
We at Novus Biologicals have added several new products to our CD antibody database. The CD, or Cluster of Differential proteins are a family of type I transmembrane glycoproteins widely expressed in immune cell populations. These include B cells, thy...  Read full blog post.

Customers Who Bought This Also Bought

IL-6 [Unconjugated]
7270-IL-025/CF

Contact Information

Product PDFs

Review this Product

Be the first to review our Human CD44 ELISA Kit (Colorimetric) and receive a gift card or discount.

Bioinformatics

Gene Symbol CD44
Entrez