CD43/Sialophorin Antibody (SP55)

Images

 
Western Blot: CD43/Sialophorin Antibody (SP55) [NBP1-49818] - Jurkat
Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (SP55) [NBP1-49818] - Human Tonsil
Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (SP55) [NBP1-49818] - CD43

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clone
SP55
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CD43/Sialophorin Antibody (SP55) Summary

Immunogen
A synthetic peptide derived from the human CD43
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
SPN
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This product is useful for Immunohistochemistry Paraffin. Antigen retrieval can be performed using a Citrate process.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
7.0 ml prediluted antibody with 10mM PBS (pH 7.4) and 1.0% BSA
Preservative
Sodium Azide
Purity
Protein A or G purified

Notes

For a positive control use Colon carcinoma.

Alternate Names for CD43/Sialophorin Antibody (SP55)

  • CD43 antigen
  • CD43
  • CD43)
  • Galactoglycoprotein
  • GALGP
  • GPL115
  • Leukocyte sialoglycoprotein
  • Leukosialin
  • LSN
  • Ly-48
  • sialophorin (gpL115, leukosialin, CD43)
  • Sialophorin
  • SPN

Background

This antibody reacts with mouse CD43, also known as leukosialin and sialophorin. CD43 has glycoforms of approximately 115 and 130 kDa, and is expressed by an early B cell subset in BM, thymocytes and mature T cells, NK cells, myeloid lineage cells and platelets. CD43 is reported to bind to CD54 and play a role in adhesion and B cell survival. While the S7 and 1B11 monoclonal antibody clones detect only certain isoforms of CD43, data suggests that R2/60 detects the expression of both isoforms. It has been demonstrated that R2/60 stains the majority of CD4+ and CD8+ splenocytes, as well as a significant population of CD19+ splenocytes. However, the clones S7 and 1B11 stain only CD4+ and CD8+, or CD19+ splenocytes, respectively. This suggests that S7 and 1B11 recognize distinct epitopes which are differentially expressed by splenic B and T cells, whereas R2/60 recognizes an epitope shared by both isoforms of CD43.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SPN
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