CD43/Sialophorin Antibody (MEM-59) [FITC]

Images

 
Western Blot: CD43/Sialophorin Antibody (MEM-59) [FITC] [NB500-385] - Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-human CD43 (MEM-59).Lane ...read more
Immunohistochemistry: CD43/Sialophorin Antibody (MEM-59) [FITC] [NB500-385] - Immunohistochemistry staining of human spleen (paraffin sections)
Flow Cytometry: CD43/Sialophorin Antibody (MEM-59) [FITC] [NB500-385] - Surface staining of human peripheral blood with anti-CD43 (MEM-59) biotin / streptavidin-APC.

Product Details

Summary
Product Discontinued
View other related CD43/Sialophorin Primary Antibodies

Order Details


    • Catalog Number
      NB500-385
    • Availability
      Product Discontinued

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CD43/Sialophorin Antibody (MEM-59) [FITC] Summary

Immunogen
Human T lymphocytes.
Specificity
The antibody CD43/Sialophorin MEM-59 recognizes neuraminidase-sensitive epitope on CD43/Sialophorin (Leukosialin), a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. CD43/Sialophorin is expressed by platelets and at high levels on the surface of all leukocytes; it is negative on resting B lymphocytes and erythrocytes.
HLDA IV; WS Code NL 604
HLDA V; WS Code AS S290
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SPN
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
Application Notes
The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 106 cells in a suspension.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS and 0.2% (w/v) high grade BSA (protease free)
Preservative
15mM Sodium Azide
Purity
Protein A or G purified

Alternate Names for CD43/Sialophorin Antibody (MEM-59) [FITC]

  • CD43 antigen
  • CD43
  • CD43)
  • Galactoglycoprotein
  • GALGP
  • GPL115
  • Leukocyte sialoglycoprotein
  • Leukosialin
  • LSN
  • Ly-48
  • sialophorin (gpL115, leukosialin, CD43)
  • Sialophorin
  • SPN

Background

CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumour suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. It appears to be an important modulator of leukocyte functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SPN
Entrez