CD43/Sialophorin Antibody (L11/135)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-65356
    • Availability
      Product Discontinued

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CD43/Sialophorin Antibody (L11/135) Summary

Immunogen
Glycoproteins from the RL-5 T-cell line
Specificity
NB100-65356 recognizes all T cells by flow cytometry; monocytes and macrophages stain weakly. Good staining of T-Cell dependent areas in lymphoid tissue sections is observed. This antibody immunoprecipitates and immunoblots a 120kD protein from RL-5 cells and T lymphocytes. It is believed to recognize the homologue of Human CD43, Leukosialin. Clone L11/135 has been reported as being suitable for use in Western blotting.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SPN
Purity
Tissue culture supernatant
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Applications/Dilutions

Dilutions
  • Flow Cytometry Neat
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Reactivity Notes

Reacts with Rabbit.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2M Tris/HCl (pH 7.4) and 5 - 10% fetal Bovine serum (FBS)
Preservative
0.09% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for CD43/Sialophorin Antibody (L11/135)

  • CD43 antigen
  • CD43
  • CD43)
  • Galactoglycoprotein
  • GALGP
  • GPL115
  • Leukocyte sialoglycoprotein
  • Leukosialin
  • LSN
  • Ly-48
  • sialophorin (gpL115, leukosialin, CD43)
  • Sialophorin
  • SPN

Background

CD43 is one of the major glycoproteins of thymocytes and T lymphocytes, it plays a role in the physicochemical properties of the T cell surface and in lectin binding. CD43 presents carbohydrate ligands to selectins. It has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. The antigen is a counter receptor for SN/Siglec1. During T cell activation CD43 is actively removed from the T cell antigen presenting cell contact site suggesting a negative regulatory role in adaptive immune response. CD43 is expressed by the majority of leucocytes, with the exception of most B cells. However CD43 expression is seen in some cases of B cell lymphocytic lymphoma and centrocytic lymphoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SPN
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