CD42b/GPIb alpha Antibody (VM16d) - BSA Free

Product Discontinued

CD42b/GPIb alpha Antibody (VM16d) - BSA Free Summary

• Immunogen: The antibody reacts with the CD42b antigen.
• Specificity: VM16d inhibits low conc. thrombin (<0.1 U/ml) induced platelet aggregation and decreases thrombin binding to platelets. F(ab')2 and F(ab') fragments are equally effective as whole intact antibodies. The VM16d epitope is located within the 45 kDa N-terminal domain of GPIb alpha- chain. VM16d does not effect platelet interaction with von Willebrand factor, leading to the conclusion that the antibody reacts with or nearby the thrombin receptor site.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GP1BA
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Functional
  • In vivo assay
• Application Notes: Analysis of platelet aggregation and detection of GPIb expression. The antibody is also useful for functional studies. The antibody is useful for indirect immunofluorescence staining with analysis by cyto-fluorometry or fluorescence microscopy. Use in In vivo reported in scientific literature (PMID: 31340152).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 3mM Sodium Azide
• Concentration: LYOPH
• Purity: Ammonium sulfate precipitation
• Reconstitution Instructions: Reconstitute with 1 mL sterile water.

Alternate Names for CD42b/GPIb alpha Antibody (VM16d) - BSA Free

• Antigen CD42b-alpha
• BP1BA
• BSS
• CD42b antigen
• CD42b
• CD42b-alpha
• glycoprotein Ib (platelet), alpha polypeptide
• Glycoprotein Ibalpha
• GP1B
• GP1BA
• GPIb alpha
• GP-Ib alpha
• GPIbA
• GPIb-alpha
• MGC34595
• platelet glycoprotein Ib alpha chain
• platelet membrane glycoprotein 1b-alpha subunit

Background

Glycoprotein Ib (GP Ib) is a platelet surface membrane glycoprotein composed of a heterodimer, an alpha chain and a beta chain, that are linked by disulfide bonds. The Gp Ib functions as a receptor for von Willebrand factor (VWF). The complete receptor complex includes noncovalent association of the alpha and beta subunits with platelet glycoprotein IX and platelet glycoprotein V. The binding of the GP Ib-IX-V complex to VWF facilitates initial platelet adhesion to vascular subendothelium after vascular injury, and also initiates signaling events within the platelet that lead to enhanced platelet activation, thrombosis, and hemostasis. This gene encodes the alpha subunit. Several polymorphisms and mutations have been described in this gene, some of which are the cause of Bernard-Soulier syndromes and platelet-type von Willebrand disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD42b/GPIb alpha Antibody (NBP1-42151)(1)

Publication using NBP1-42151

• Carestia A, Mena HA, Olexen CM et al. Platelets Promote Macrophage Polarization toward Pro-inflammatory Phenotype and Increase Survival of Septic Mice Cell Rep 2019-07-23 [PMID: 31340152] (In Vivo)

Bioinformatics

• Gene Symbol: GP1BA