Recombinant Human CD40 Ligand/TNFSF5 Protein

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Summary
Product Discontinued
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    • Catalog Number
      NBP1-99356
    • Availability
      Product Discontinued

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Recombinant Human CD40 Ligand/TNFSF5 Protein Summary

Description
A recombinant protein corresponding to 149 amino acids of Human CD40 Ligand/TNFSF5
Preparation
Method
>98% SDS-PAGE
Details of Functionality
Biological Activity: Determined by the stimulation of IL-12 induction by human peripheral blood mononuclear cells (PBMC) and the stimulation of IL-8 induction by human PBMC. The expected ED50 for this effect is 5-10 ng/ml. Note: results may vary with PBMC donors.
Results: 5-10 ng/ml
Protein/Peptide Type
Recombinant Protein
Gene
CD40LG
Purity
>90% SDS-PAGE
Endotoxin Note
<0.1 ng/ug

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
From 10 mM Sodium Phosphate, pH 7.5.
Concentration
Lyoph
Purity
>90% SDS-PAGE
Reconstitution Instructions
Reconstitute with 5 - 10 mM Sodium Phosphate (pH 7.5) to a final concentration of 0.1 - 1.0 mg/ml.

Notes

After reconstituted, dilute further with other aqueous solutions.

Alternate Names for Recombinant Human CD40 Ligand/TNFSF5 Protein

  • CD154 antigen
  • CD154
  • CD40 antigen ligand
  • CD40 Ligand
  • CD40-L
  • CD40LG
  • CD40LIGM
  • gp39
  • hCD40L
  • HIGM1
  • T-B cell-activating molecule
  • T-BAM
  • T-cell antigen Gp39
  • TNF-related activation protein
  • TNFSF5
  • TNFSF5IMD3
  • TRAP
  • TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
  • tumor necrosis factor (ligand) superfamily member 5
  • Tumor necrosis factor ligand superfamily member 5

Background

CD40 Ligand (CD40L/TRAP) is a type II membrane protein that effectuates the helper function of T cells on resting B cells. 's Human soluble CD40 Ligand is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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FAQs for CD40 Ligand/TNFSF5 Protein (NBP1-99356). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for paired ABs for use in detecting CD40L (rat) by ELISA. Do you have such a reagent(s) available?
    • Unfortunately we currently do not have any CD40 Ligand antibody pairs that have been validated in Rat. The only antibody pairs we offer for CD40L are validated in Human. I apologize for this inconvenience. If you would be interested in testing one of our products in Rat I'd like to invite you to participate in our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

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Bioinformatics

Gene Symbol CD40LG
Entrez