CD4 Antibody (RPA-T4) [PE]

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Flow Cytometry: CD4 Antibody (RPA-T4) [PE] [NB100-63755] - Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:RPE
Flow Cytometry: CD4 Antibody (RPA-T4) [PE] [NB100-63755] - Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD4:RPE

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow
Clone
RPA-T4
Clonality
Monoclonal
Host
Mouse
Conjugate
PE

Order Details

CD4 Antibody (RPA-T4) [PE] Summary

Immunogen
Human PHA blasts
Specificity
NB100-64882 recognizes human CD4, a 55kD cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages. Epitope mapping studies have shown that antibodies, produced by clone RPA-T4, recognize an epitope within domain 1, of the extracellular region, of the CD4 molecule. Clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD4
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
For immunofluorescent staining, the suggested use of this reagent is: 0.25 microgram per one million cells in 100 microliter volume(PMID: 21520247). For IHC-frozen sections, it is recommended that the reagent be titrated for optimal performance for each application.
The antibody works well on acetone-fixed frozen sections. It sometime works on formalin-fixed paraffin-embedded tissue sections but inconsistent results have been reported. The RM4-5 antibody blocks the binding of GK1.5 antibody (NBP1-26692) to CD4 T cells, but not RM4-4 antibody (NB100-77603).

Reactivity Notes

Reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 1.0% BSA, and 5% Sucrose
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for CD4 Antibody (RPA-T4) [PE]

  • CD4 antigen (p55)
  • CD4 antigen
  • CD4 molecule
  • CD4 receptor
  • CD4
  • CD4mut
  • T-cell surface antigen T4/Leu-3
  • T-cell surface glycoprotein CD4

Background

CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for CD4 Antibody (NB100-63755). (Showing 1 - 1 of 1 FAQ).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies

 

Isotype Controls

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Blogs on CD4. Showing 1-10 of 14 blog posts - Show all blog posts.

The role of MHC Class II RT1B and immune response post brain injury
The major histocompatibility complex (MHC) is responsible for binding peptide fragments arising from pathogens in order to display them on the cell surface for recognition from immune cells.  Once recognized, the foreign pathogen is typically evade...  Read full blog post.

Topics in CD11b: The innate immune response
Integrins are transmembrane receptors composed of alpha and beta chains, where beta-integrins are mainly expressed in leukocytes. Leukocytes are white blood cells that act in the immune system to defend our body against foreign pathogens.  Integrin...  Read full blog post.

Transferrin and the blood brain barrier
Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

CD3 (OKT3) as a marker of immune response efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.

CD20 (Cluster of differentiation 20, Membrane-spanning 4-domains subfamily A member 1 (MS4A1), CVID5, B-lymphocyte surface antigen B1)
CD20 is a human B-lymphocyte surface molecule that spans the membrane four times and is expressed on both normal and malignant cells. The CD20 antigen displays a unique expression pattern among hematopoietic cells - it is present on human pre B-ly...  Read full blog post.

CD8 alpha - Marker for cytotoxic T lymphocytes
The T-cell co-receptor CD8 is a cell-surface glycoprotein that bridges the lipid bilayer by interacting externally with ligands, such as major histocompatibility complex (MHC) class I, as well as internally with signaling molecules such as tyrosine ki...  Read full blog post.

Lymphocyte activation gene 3 (LAG3): No lag time in immune response
The LAG3 protein belongs to the Ig superfamily and contains 4 extracellular Ig-like domains (D1-D4). This molecule plays an key role in the immune response through negative regulation of T-cell proliferation, function, and homeostasis. It is required...  Read full blog post.

CD4 - An Important Co-receptor Assisting TCRs
The cluster of differentiation 4 (CD4) protein is a surface, type I membrane-embedded glycoprotein that is found on a wide range of cells: T-lymphocytes, B-cells, macrophages, granulocytes, and developmentally-dependent regions specific to the brain....  Read full blog post.

CD4, HIV and T Cell Signaling
CD4, also known as Cluster of Differentiation 4, interacts with major histocompatibility complex class II antigens, acts as a receptor for the human immunodeficiency virus and induces the aggregation of lipid rafts. It is expressed in T lymphocytes, B...  Read full blog post.

Showing 1-10 of 14 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol CD4
Entrez
Uniprot

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