CD4 Antibody (3-4F4) [FITC]

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Flow Cytometry: CD4 Antibody (3-4F4) [FITC] [NBP1-28259] - 1 ug/10^6 Peripheral blood mononuclear cells from a healthy domestic cat were double-stained with mouse anti-feline CD4-FITC and mouse anti-feline CD8-R-PE. ...read more

Product Details

Summary
Reactivity FeSpecies Glossary
Applications Flow, IHC, IHC-Fr, IP, ICC/IF
Clone
3-4F4
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

Order Details

CD4 Antibody (3-4F4) [FITC] Summary

Immunogen
Feline T cells
Specificity
Feline CD4, Mr 65 kDa
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD4
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry <= 1 ug/10^6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Immunocytochemistry
Reviewed Applications
Read 1 Review rated 5
using
NBP1-28259 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for CD4 Antibody (3-4F4) [FITC]

  • CD4 antigen (p55)
  • CD4 antigen
  • CD4 molecule
  • CD4 receptor
  • CD4
  • CD4mut
  • T-cell surface antigen T4/Leu-3
  • T-cell surface glycoprotein CD4

Background

Feline CD4, a member of the immunoglobulin superfamily of cell surface receptors, is a type I transmembrane glycoprotein that is expressed on the 'helper/inducer' subpopulation of peripheral T lymphocytes. It is present on approximately 55% of thymocytes, 15% of splenocytes, 40% of lymph node cells, and 25% of peripheral blood lymphocytes. (1-3) CD4 functions as an accessory molecule in the recognition of foreign antigens in association with MHC Class II antigens by T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD4 Antibody (NBP1-28259) (0)

There are no publications for CD4 Antibody (NBP1-28259).
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Review for CD4 Antibody (NBP1-28259) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Feline.

Reviews using NBP1-28259:
Filter by Applications
Flow
(1)
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Filter by Species
Feline
(1)
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Images Ratings Applications Species Date Details
Flow Cytometry CD4 NBP1-28259
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reviewed by:
A G
Flow Feline 03/17/2017
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Summary

ApplicationFlow Cytometry
Sample TestedWhole blood cells
SpeciesFeline
LotJ1507-w375c

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD4 Antibody (NBP1-28259). (Showing 1 - 1 of 1 FAQ).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NBP1-28258APC
Biotin Labeled NBP1-28260
DyLight 350 Labeled NBP1-28258UV
DyLight 405 Labeled NBP1-28258V
DyLight 488 Labeled NBP1-28258G
DyLight 680 Labeled NBP1-28258FR
DyLight 755 Labeled NBP1-28258IR
PE Labeled NBP1-28258PE
PerCP Labeled NBP1-28258PCP

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Blogs on CD4. Showing 1-10 of 17 blog posts - Show all blog posts.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein...  Read full blog post.

Flow Cytometry Basics for the Non-Expert
By Jody Bonnevier, PhD.Flow cytometry: A Challenging Interdisciplinary ApplicationFlow cytometry has long been the cell analysis method of choice for immunologists, however more and more disciplines have recognize...  Read full blog post.

The role of MHC Class II RT1B and immune response post brain injury
The major histocompatibility complex (MHC) is responsible for binding peptide fragments arising from pathogens in order to display them on the cell surface for recognition from immune cells.  Once recognized, the foreign pathogen is typically evade...  Read full blog post.

Topics in CD11b: The innate immune response
Integrins are transmembrane receptors composed of alpha and beta chains, where beta-integrins are mainly expressed in leukocytes. Leukocytes are white blood cells that act in the immune system to defend our body against foreign pathogens.  Integrin...  Read full blog post.

Transferrin and the blood brain barrier
Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

CD3 (OKT3) as a marker of immune response efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.

CD20 (Cluster of differentiation 20, Membrane-spanning 4-domains subfamily A member 1 (MS4A1), CVID5, B-lymphocyte surface antigen B1)
CD20 is a human B-lymphocyte surface molecule that spans the membrane four times and is expressed on both normal and malignant cells. The CD20 antigen displays a unique expression pattern among hematopoietic cells - it is present on human pre B-ly...  Read full blog post.

CD8 alpha - Marker for cytotoxic T lymphocytes
The T-cell co-receptor CD8 is a cell-surface glycoprotein that bridges the lipid bilayer by interacting externally with ligands, such as major histocompatibility complex (MHC) class I, as well as internally with signaling molecules such as tyrosine ki...  Read full blog post.

Showing 1-10 of 17 blog posts - Show all blog posts.

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Recent Reviews

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A G
03/17/2017
Application: Flow
Species: Feline

Bioinformatics

Gene Symbol CD4