CD36/SR-B3 Antibody [FITC]

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Product Details

Summary
Reactivity Hu, Mu, Rt, SyHa, Pm, RbSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
FITC

CD36/SR-B3 Antibody [FITC] Summary

Immunogen
A synthetic peptide mapping to a region of human CD36 between residues 300-400. [UniProt# P16671]
Localization
Integral membrane
Marker
Endothelial Cell Marker
Predicted Species
Rat (95%), Golden Syrian Hamster (95%), Primate (95%), Rabbit (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD36
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
Application Notes
This CD36 antibody is useful for Western blot, where a band is seen ~75-80 kDa. May see a very faint band ~130 kDa with a flash exposure and several bands with exposure times longer than 3 seconds. The theoretical molecular weight of CD36 is ~53 kDa. The difference in theoretical MW and actual MW as seen in Western blot is most likely due to the heavy glycosylation and palmitoylation of this protein.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human.Mouse reactivity reported in scientific literature (PMID: 24163073) Immunogen sequence has 95% homology to rat, monkey, Golden hamster and rabbit, 90% homology to mouse, and 75% homology to bovine.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CD36/SR-B3 Antibody [FITC]

  • CD36 antigen
  • CD36 molecule (thrombospondin receptor)
  • CD36
  • Collagen R
  • FAT
  • FATCHDS7
  • Fatty acid translocase
  • Glycoprotein IIIb
  • GP3Bthrombospondin receptor)
  • GPIIIb
  • GPIV
  • Leukocyte differentiation antigen CD36
  • PAS IV
  • PAS-4
  • Platelet collagen receptor
  • platelet glycoprotein 4
  • Platelet glycoprotein IV
  • SCARB3
  • scavenger receptor class B, member 3
  • SRB3
  • SR-B3
  • Thrombospondin R
  • Thrombospondin receptor

Background

CD36 (leukocyte differentiation antigen CD36), a multifunctional protein homologous to SR-B1, is found in tissues with sustained lipid metabolism and in hematopoieic cells such as microvascular endothelium, phagocytes (macrophages, dendritic cells, microglia, and retinal pigment epithelium), erythroid precursors, hepatocytes, adipocytes, cardiac and skeletal myocytes, and specialized epithelium of the breast, kidney, gut etc. CD36 act as a negative regulator of angiogenesis via its function as a receptor for thrombospondin-1 (THBS1) and related proteins on endothelial cells. On phagocytes, through its ability to recognize specific oxidized phospholipids and lipoproteins, CD36 participates in internalization of apoptotic cells, certain bacterial/fungal pathogens, and modified LDLs, thus contributing to inflammatory responses and atherothrombotic diseases. CD36 binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption, thereby contributing to pathogenesis of diabetes/obesity. Moreover, genetic variations in CD36 are involved in susceptibility to malaria, and defects in CD36 are the cause of platelet glycoprotein IV deficiency, also called CD36 deficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD36
Entrez