CD31/PECAM-1 Antibody

Images

 
Western Blot: CD31/PECAM1 Antibody [NBP2-15797] - Sample (30 ug of whole cell lysate) A: THP-1 7. 5% SDS PAGE gel, diluted at 1:10000.
Immunohistochemistry-Paraffin: CD31/PECAM1 Antibody [NBP2-15797] - Immunohistochemical analysis of paraffin-embedded Lung ca Macrophage endothelial cell, using antibody at 1:250 dilution.

Product Details

Summary
Product Discontinued
View other related CD31/PECAM-1 Primary Antibodies

Order Details


    • Catalog Number
      NBP2-15797
    • Availability
      Product Discontinued

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CD31/PECAM-1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CD31. The exact sequence is proprietary.
Localization
Membrane; Single-pass type I membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PECAM1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CD31/PECAM-1 Antibody

  • adhesion molecule
  • CD31 antigen
  • CD31
  • CD31/EndoCAM
  • endoCAM
  • FLJ34100
  • FLJ58394
  • GPIIA'
  • PECA1
  • PECAM1
  • PECAM-1
  • PECAM-1, CD31/EndoCAM
  • platelet endothelial cell adhesion molecule
  • platelet/endothelial cell adhesion molecule

Background

Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for CD31/PECAM-1 Antibody (NBP2-15797). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for references concerning the use of NBP2-15797, NBP1-47913 and NB100-2284 in IHC on PFA- fixed section.
    • Among your mentioned catalog numbers (NBP2-15797, NBP1-47913 and NB100-2284), we have published references for NB100-2284 only. Hylander et al. 2013 (Journal of Translational Medicine 2013, 11:110) have cited the IHC-P use of NB100-2284 and this paper has also explained a nice detailed IHC-P protocol that you may follow if you want to. NBP1-71663 is our bestselling catalog number among all CD31/PECAM1 antibodies and it has been cited in two publications, both explaining its use in IHC-P assays (Pratheeshkumar et al. 2012 PLoS One. 2012;7(10):e47516; and Pratheeshkumar et al. 2012 PLoS One. 2012;7(12):e52279)

Secondary Antibodies

 

Isotype Controls

Additional CD31/PECAM-1 Products

Bioinformatics Tool for CD31/PECAM-1 Antibody (NBP2-15797)

Discover related pathways, diseases and genes to CD31/PECAM-1 Antibody (NBP2-15797). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for CD31/PECAM-1 Antibody (NBP2-15797)

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Pathways for CD31/PECAM-1 Antibody (NBP2-15797)

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PTMs for CD31/PECAM-1 Antibody (NBP2-15797)

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Research Areas for CD31/PECAM-1 Antibody (NBP2-15797)

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Blogs on CD31/PECAM-1.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

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Bioinformatics

Gene Symbol PECAM1
Entrez
Uniprot