CD31/PECAM-1 Antibody (VM64)

Images

 

Product Details

Summary
Product Discontinued
View other related CD31/PECAM-1 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-42152
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

CD31/PECAM-1 Antibody (VM64) Summary

Immunogen
The antibody reacts with platelets, monocytes/macrophages, granulocytes and B-cells.
Specificity
The antibody reacts with platelets, monocytes/macrophages, granulocytes and B-cells.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PECAM1
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
It stains the cell boundaries of endothelial cells with a staining pattern identical to that of silverstaining. Furthermore in histology it stains weakly histocytes and glomeruli. Useful for functional studies on cell-cell interactions; studies on integrity of endothelial cell monolayers; and the antibody can be used for immunoblotting under non reducing conditions.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-42152 in the following applications:

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
Sodium Azide
Concentration
LYOPH
Purity
Ammonium sulfate precipitation
Reconstitution Instructions
Reconstitute with 1.0 ml sterilized water.

Alternate Names for CD31/PECAM-1 Antibody (VM64)

  • adhesion molecule
  • CD31 antigen
  • CD31
  • CD31/EndoCAM
  • endoCAM
  • FLJ34100
  • FLJ58394
  • GPIIA'
  • PECA1
  • PECAM1
  • PECAM-1
  • PECAM-1, CD31/EndoCAM
  • platelet endothelial cell adhesion molecule
  • platelet/endothelial cell adhesion molecule

Background

CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells and is specifically concentrated at the junction between them. Therefore, CD31 antibodies are commonly used as endothelial markers to measure angiogenesis in association with tumor recurrence. It is also weakly expressed on many peripheral lymphoid cells and platelets. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas). It is also weakly expressed on many peripheral lymphoid cells and platelets.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB600-1071
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
NB600-586
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB100-77417
Species: Mu
Applications: WB, Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vivo, CyTOF-ready
AF1320
Species: Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
AF644
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready, Neut
NB300-223
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-91761
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-42152
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr

Publications for CD31/PECAM-1 Antibody (NBP1-42152) (0)

There are no publications for CD31/PECAM-1 Antibody (NBP1-42152).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for CD31/PECAM-1 Antibody (NBP1-42152) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-42152:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 12/28/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman
CommentsThe signal for this Ab is strong and clear. Also tested species in IHC-Fr application yielding similar results.

Blocking

Blocking DetailsBlocking Buffer: 1% BSA

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 100x dilution, Primary Ab Incubation Time: 1 hour, Primary Ab Incubation Temp: room temperature

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat anti-rabbit IgG Dylight 649

Details

Detection NotesDetection Method: Image Quant, Confocul Microscopy

Comments

CommentsThe signal for this Ab is strong and clear. Also tested species in IHC-Fr application yielding similar results.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD31/PECAM-1 Antibody (NBP1-42152). (Showing 1 - 1 of 1 FAQ).

  1. Can this primary antibody be used with an IgG2b secondary (as opposed to the more specific IgG2b kappa)?
    • Yes, this antibody will be detected by an IgG2b secondary antibody.

Secondary Antibodies

 

Isotype Controls

Additional CD31/PECAM-1 Products

Bioinformatics Tool for CD31/PECAM-1 Antibody (NBP1-42152)

Discover related pathways, diseases and genes to CD31/PECAM-1 Antibody (NBP1-42152). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CD31/PECAM-1 Antibody (NBP1-42152)

Discover more about diseases related to CD31/PECAM-1 Antibody (NBP1-42152).
 

Pathways for CD31/PECAM-1 Antibody (NBP1-42152)

View related products by pathway.

PTMs for CD31/PECAM-1 Antibody (NBP1-42152)

Learn more about PTMs related to CD31/PECAM-1 Antibody (NBP1-42152).
 

Research Areas for CD31/PECAM-1 Antibody (NBP1-42152)

Find related products by research area.

Blogs on CD31/PECAM-1.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

 
Anonymous
12/28/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol PECAM1
Entrez
Uniprot