CD300e/LMIR6 Antibody (UP-H2)

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Flow Cytometry: CD300e/LMIR6 Antibody (UP-H2) [NBP1-43325] - Staining of normal human peripheral blood cells with 0.06 micrograms of Mouse IgG1 k Isotype Control Purified (blue histogram) or 0.06 micrograms of ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-43325
    • Availability
      Product Discontinued

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CD300e/LMIR6 Antibody (UP-H2) Summary

Immunogen
The immunogen for this antibody was IREM2.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD300E
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 1:10-1:500
Application Notes
This UP-H2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.125 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD300e/LMIR6 Antibody (UP-H2)

  • CD300 antigen like family member E
  • CD300 antigen-like family member E
  • CD300e antigenPIgR-2
  • CD300e molecule
  • CD300e
  • CD300LE
  • CD300LEPIgR2
  • CLM2
  • CLM-2
  • CLM2CMRF35-A5
  • CMRF35A5
  • CMRF35-like molecule 2
  • Immune receptor expressed on myeloid cells 2
  • IREM-2
  • IREM2IREM-2
  • LMIR6
  • Poly-Ig receptor 2
  • Polymeric immunoglobulin receptor 2

Background

The monoclonal antibody UP-H2 recognizes human IREM-2 (immune receptor expressed in monocytic derived cells) also known as LMIR6, and unofficially CD300E and CD300LE. It is a member of the CMRF-35 family. This 34 kDa Ig-like containing receptor is an activating receptor and through its charged residues in the cytokplasmic domain interacts with ITAM containing adaptor proteins like DAP12. Expression is found exclusively on monocytes and myeloid DCs. Expression may be transient as differentiation of monocytes leads to down regulation of IREM-2 yet expression is found on mature DCs. It has been also noted that myeloid cell lines such as THP-1 and U937 do not express IREM-2. In vitro addition of UP-H2 antibody either soluble or crosslinked stimulates monocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD300E
Entrez