CD30/TNFRSF8 Antibody (15B3)

Product Discontinued

CD30/TNFRSF8 Antibody (15B3) Summary

• Immunogen: Recombinant human CD30 antigen
• Specificity: Recognizes the human CD30 cell surface antigen, a 120kD glycoprotein. CD30 is expressed by some activated T lymphocytes, and also by Reed Sternberg cells of Hodgkin's disease and in cases of anaplastic large cell lymphoma.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFRSF8
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:20-1:40
  • Immunohistochemistry-Paraffin 1:20-1:40
• Application Notes: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium Citrate buffer pH6.0 is recommended for this purpose.
• Control:

  CD30/TNFRSF8 Overexpression Lysate

Reactivity Notes

Reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: No buffer
• Preservative: 0.09% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for CD30/TNFRSF8 Antibody (15B3)

• CD30 antigen
• CD30
• CD30KI-1
• CD30L receptor
• cytokine receptor CD30
• D1S166EKi-1
• Ki-1 antigen
• Lymphocyte activation antigen CD30
• TNFRSF8
• tumor necrosis factor receptor superfamily member 8
• tumor necrosis factor receptor superfamily, member 8

Background

CD30 is a type I transmembrane glycoprotein of the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkin's and Reed-Sternberg cells using monoclonal antibody Ki-1. The ligand for CD30 is CD30L (CD153). The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. CD30 has a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. In addition to its expression on Hodgkin's and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin's lymphomas (including Burkitt's lymphomas), virus-infected T and B cells, and on normal T and B cells after activation. In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that coexpress CD45RO and the IL4 receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNFRSF8
• Entrez:
  • 943(Human)
• Uniprot:
  • P28908()