CD28 Antibody (PV-1)

Images

 
Flow Cytometry: CD28 Antibody (PV-1) [NBP1-27932] - Analysis using the PE conjugate of NBP1-27932. Staining of BALB/cAnN mouse b lymphocyte cell line A20 and C57BL/6N lymphocyte cell line EL-4.
Flow Cytometry: CD28 Antibody (PV-1) [NBP1-27932] - Analysis using the Allophycocyanin conjugate of NBP1-27932. Staining of BALB/cAnN B lymphocyte cell line A20 adn C57BL/6N T lymphocytes cell line EL-4.
Flow Cytometry: CD28 Antibody (PV-1) [NBP1-27932] - Analysis using the Biotin conjugate of NBP1-27932. Staining of BALB/cAnN B lymphocyte cell line A20 and C57BL/6N T lymphocyte cell line EL-4.
Flow Cytometry: CD28 Antibody (PV-1) [NBP1-27932] - Analysis using the FITC conjugate of NBP1-27932. Staining of 3 ug/1^6 cells A 50:50 mixture of the A20 B-cell lymphoma and the EL-4 T-cell lymphoma with hamster ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, In vitro, IP
Clone
PV-1
Clonality
Monoclonal
Host
Armenian Hamster
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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Catalog# & Conjugate Size Price

CD28 Antibody (PV-1) Summary

Immunogen
C57BL/6N mouse T cell lymphoma EL4 cell line
Specificity
Murine CD28
Isotype
IgG
Clonality
Monoclonal
Host
Armenian Hamster
Gene
CD28
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10 - 1:1000
  • Immunoprecipitation 1:10 - 1:500
  • In vitro assay

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD28 Antibody (PV-1)

  • CD28 antigen (Tp44)
  • CD28 antigen
  • CD28 molecule
  • CD28
  • MGC138290
  • T-cell-specific surface glycoprotein CD28
  • Tp44

Background

CD28 is a type I disulfide-linked homodimer that is constitutively expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T lymphocytes, and at very low levels on NK cells. Its expression is upregulated upon T-cell activation. (1-4) CD28 is a ligand for CD80/B7-1 and CD86/B7-2 on B cells and other antigen presenting cells, and plays an important role in the interaction between T cells and B cells. (5) CD28 is a co-stimulatory receptor involved in many, but not all, T-cell independent immune responses. (6-9)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for CD28 Antibody (NBP1-27932) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NBP1-27936
Biotin Labeled NBP1-27934
FITC Labeled NBP1-27933
PE Labeled NBP1-27935

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Bioinformatics Tool for CD28 Antibody (NBP1-27932)

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Research Areas for CD28 Antibody (NBP1-27932)

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Blogs on CD28.

Thomson Reuters Predicts 2016 Nobel Prize Winners
Here at Bio-Techne we always look forward to the annual announcements of winners of the highly coveted Nobel Prize – the greatest award in science. How can you go about predicting which scientists might be in line for a life-changing phone-call fro...  Read full blog post.

CD80: A co-stimulator of T cell activation
CD80 is a 60kD single chain type I transmembrane glycoprotein that is a member of the immunoglobulin family. CD80 is expressed on activated B- and T-lymphocytes, as well as a subpopulation of previously activated B-cells, but not on the majority of re...  Read full blog post.

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Bioinformatics

Gene Symbol CD28
Entrez