CD28 Antibody (CD28.2)

Images

 
Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining pattern of human peripheral blood stained using anti-human CD28 (CD28.2) purified antibody (concentration in sample 1,6 ug/ml) GAM APC.
Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) purified.
Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PE.
Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PerCP-CyTM5.5
Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) APC.
Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PerCP.
Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) FITC.
Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PE-CyTM7.
Flow Cytometry: CD28 Antibody (CD28.2) [NBP1-79126] - Separation of human CD28 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IP, CyTOF-ready
Clone
CD28.2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CD28 Antibody (CD28.2) Summary

Immunogen
DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA
Specificity
The antibody CD28.2 reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells. HLDA V.; WS Code 5T CD28.05
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD28
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1-12 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunoprecipitation 1:50
  • CyTOF-ready
Application Notes
This antibody is Cytof ready.
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CD28 Knockout HeLa Cell Lysate
Publications
Read Publication using
NBP1-79126 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for CD28 Antibody (CD28.2)

  • CD28 antigen (Tp44)
  • CD28 antigen
  • CD28 molecule
  • CD28
  • MGC138290
  • T-cell-specific surface glycoprotein CD28
  • Tp44

Background

CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD28 Antibody (NBP1-79126)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD28 Antibody (NBP1-79126) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-79126AF350
Alexa Fluor 405 NBP1-79126AF405
Alexa Fluor 488 NBP1-79126AF488
Alexa Fluor 532 NBP1-79126AF532
Alexa Fluor 594 NBP1-79126AF594
Alexa Fluor 647 NBP1-79126AF647
Alexa Fluor 700 NBP1-79126AF700
Alexa Fluor 750 NBP1-79126AF750
Allophycocyanin NBP1-79126APC
Allophycocyanin/Cy7 NBP1-79126APCCY7
Biotin NBP1-79126B
DyLight 350 NBP1-79126UV
DyLight 405 NBP1-79126V
DyLight 488 NBP1-79126G
DyLight 550 NBP1-79126R
DyLight 594 NBP1-79126DL594
DyLight 650 NBP1-79126C
DyLight 680 NBP1-79126FR
DyLight 755 NBP1-79126IR
HRP NBP1-79126H
Janelia Fluor 549 NBP1-79126JF549
Janelia Fluor 646 NBP1-79126JF646
PE NBP1-79126PE
PE/Atto594 NBP1-79126PEATT594
PE/Cy5.5 NBP1-79126PECY55
PE/Cy7 NBP1-79126PECY7
PerCP NBP1-79126PCP

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Here at Bio-Techne we always look forward to the annual announcements of winners of the highly coveted Nobel Prize – the greatest award in science. How can you go about predicting which scientists might be in line for a life-changing phone-call fro...  Read full blog post.

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CD80 is a 60kD single chain type I transmembrane glycoprotein that is a member of the immunoglobulin family. CD80 is expressed on activated B- and T-lymphocytes, as well as a subpopulation of previously activated B-cells, but not on the majority of re...  Read full blog post.

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Bioinformatics

Gene Symbol CD28
Entrez
Uniprot