CD28 Antibody (AV7)

Images

 
Flow Cytometry: CD28 Antibody (AV7) [NBP1-28291] - Analysis using the PE conjugate of NBP1-28291. Multiple staining of peripheral blood mononuclear cells.
Flow Cytometry: CD28 Antibody (AV7) [NBP1-28291] - Analysis using the Biotin conjugate of NBP1-28291. Multiple staining of peripheral blood mononuclear cells.
Flow Cytometry: CD28 Antibody (AV7) [NBP1-28291] - Analysis using the FITC conjugate of NBP1-28291. Double staining of Chicken peripheral blood mononuclear cells with mouse anti-chicken CD28-FITC and mouse anti-CD3-PE.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-28291
    • Availability
      Product Discontinued

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CD28 Antibody (AV7) Summary

Immunogen
Chicken peripheral blood lymphocytes
Specificity
Chicken CD28, a monomeric molecule of Mr 40-44 kDa with 50% amino acid sequence identity with mammalian CD28
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD28
Purity
IgM purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10 - 1:1000
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10 - 1:500
  • In vitro assay
  • Immunocytochemistry

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
IgM purified

Alternate Names for CD28 Antibody (AV7)

  • CD28 antigen (Tp44)
  • CD28 antigen
  • CD28 molecule
  • CD28
  • MGC138290
  • T-cell-specific surface glycoprotein CD28
  • Tp44

Background

Chicken CD28 antigen is a type I transmembrane monomeric glycoprotein which does not contain the cysteine residues that form disulfide-linked covalent homodimers found in the mammalian counterpart. This may reduce the avidity of binding to individual ligand molecules expected to be present on Ag presenting cells. Peripheral gamma delta+ T cells are CD28-negative. (1) Monoclonal antibodies against CD28 have a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A or MAbs against the avian T cell receptor (TCR). Both Vbeta1- and Vbeta2-expressing cells respond equally well to stimulation with anti-CD28 in combination with PMA. These responses are resistant to cyclosporin A, but inhibited by herbimycin A, suggesting that CD28 employs a signalling pathway at least partly distinct from that triggered by TCR/CD3. (2)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for CD28 Antibody (NBP1-28291)

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Research Areas for CD28 Antibody (NBP1-28291)

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Blogs on CD28.

Thomson Reuters Predicts 2016 Nobel Prize Winners
Here at Bio-Techne we always look forward to the annual announcements of winners of the highly coveted Nobel Prize – the greatest award in science. How can you go about predicting which scientists might be in line for a life-changing phone-call fro...  Read full blog post.

CD80: A co-stimulator of T cell activation
CD80 is a 60kD single chain type I transmembrane glycoprotein that is a member of the immunoglobulin family. CD80 is expressed on activated B- and T-lymphocytes, as well as a subpopulation of previously activated B-cells, but not on the majority of re...  Read full blog post.

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Bioinformatics

Gene Symbol CD28
Entrez