CD28 Antibody (39482)


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Product Details

Reactivity Av, ChSpecies Glossary
Applications Flow, IHC, IHC-Fr, IP, WB (-)
1.0 mg/ml

CD28 Antibody (39482) Summary

Chicken embryonic thymocytes
Clone 2-4 (NB100-65334) was originally reported to recognize avian CD2, but has subsequently been found to recognize the 40kDa chicken CD28 homologue (2). In chickens CD28 is expressed by all T cells excluding T cells that are gamma/delta positive. Studies suggest that the co-stimulatory role of CD28 is important to T cell function in chickens.
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Phosphate buffered saline pH7.4
0.09% Sodium Azide
1.0 mg/ml
Protein G purified

Reactivity Notes

Reacts with Chicken. Cross reacts with Duck.

Alternate Names for CD28 Antibody (39482)

  • CD28 antigen (Tp44)
  • CD28 antigen
  • CD28 molecule
  • CD28
  • MGC138290
  • T-cell-specific surface glycoprotein CD28
  • Tp44


Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-65334AF405
Alexa Fluor (R) 488 Labeled NB100-65334AF488
Alexa Fluor (R) 647 Labeled NB100-65334AF647
Alexa Fluor (R) 700 Labeled NB100-65334AF700
Allophycocyanin Labeled NB100-65334APC
DyLight 350 Labeled NB100-65334UV
DyLight 405 Labeled NB100-65334V
DyLight 405LS Labeled NB100-65334V3
DyLight 488 Labeled NB100-65334G
DyLight 680 Labeled NB100-65334FR
DyLight 755 Labeled NB100-65334IR
PE Labeled NB100-65334PE
PerCP Labeled NB100-65334PCP

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Blogs on CD28.

CD80: A co-stimulator of T cell activation
CD80 is a 60kD single chain type I transmembrane glycoprotein that is a member of the immunoglobulin family. CD80 is expressed on activated B- and T-lymphocytes, as well as a subpopulation of previously activated B-cells, but not on the majority of re...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol CD28

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