CD25/IL-2 R alpha Antibody (RM134L) [PE]

Images

 
Flow Cytometry: OX40 Ligand/TNFSF4 Antibody (RM134L) [PE] [NBP2-00363] - Staining of 4-day unstimulated (orange histogram) and 4-day Anti-Mouse IgM and Anti-Mouse CD40-stimulated (purple histogram) BALB/c splenocytes ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-00363
    • Availability
      Product Discontinued

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CD25/IL-2 R alpha Antibody (RM134L) [PE] Summary

Immunogen
The immunogen for this antibody was OX40 Ligand.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Rat
Gene
IL2RA
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
The RM134L antibody has been tested by flow cytometric analysis of activated mouse splenocyte suspensions and can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for CD25/IL-2 R alpha Antibody (RM134L) [PE]

  • CD25 antigen
  • CD25
  • IDDM10
  • IL-2 R alpha
  • IL-2 receptor subunit alpha
  • IL2R alpha
  • IL-2R subunit alpha
  • IL2R
  • IL2RA
  • IL-2Ra
  • IL-2-RA
  • IL2-RA
  • interleukin 2 receptor, alpha
  • interleukin-2 receptor subunit alpha
  • p55
  • TAC antigen
  • TCGFR

Background

The RM134L monoclonal antibody reacts with mouse CD252 also known as OX-40 Ligand, a member of the TNF superfamily. OX-40L is induced on mouse splenic B cells stimulated with a combination of anti-IgM and anti-CD40. Neither resting nor activated mouse T cells express OX-40L. The interaction of OX-40 (CD134) with OX-40L has been implicated in T cell-dependent humoral response. RM134L inhibits the costimulatory activity of mouse OX-40L transfected cells for anti-CD3-stimulated T cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Additional CD25/IL-2 R alpha Products

Bioinformatics Tool for OX40 Ligand/TNFSF4 Antibody (NBP2-00363)

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Diseases for OX40 Ligand/TNFSF4 Antibody (NBP2-00363)

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Bioinformatics

Gene Symbol IL2RA
Entrez