CD25/IL-2 R alpha Antibody (RM134L)

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Flow Cytometry: CD25/IL-2 R alpha Antibody (RM134L) [NBP1-43277] - Analysis using the PE conjugate of NBP1-43277. Staining of 4-day unstimulated (orange histogram) and 4-day Anti-Mouse IgM and Anti-Mouse CD40-stimulated ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-43277
    • Availability
      Product Discontinued

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CD25/IL-2 R alpha Antibody (RM134L) Summary

Immunogen
The immunogen for this antibody was OX40 Ligand.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Rat
Gene
IL2RA
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Functional
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
The RM134L antibody has been tested by flow cytometric analysis of activated mouse splenocyte suspensions and can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Purity
Protein A or G purified

Alternate Names for CD25/IL-2 R alpha Antibody (RM134L)

  • CD25 antigen
  • CD25
  • IDDM10
  • IL-2 R alpha
  • IL-2 receptor subunit alpha
  • IL2R alpha
  • IL-2R subunit alpha
  • IL2R
  • IL2RA
  • IL-2Ra
  • IL-2-RA
  • IL2-RA
  • interleukin 2 receptor, alpha
  • interleukin-2 receptor subunit alpha
  • p55
  • TAC antigen
  • TCGFR

Background

The RM134L monoclonal antibody reacts with mouse OX-40 Ligand, a member of the TNF superfamily. OX-40L is induced on mouse splenic B cells stimulated with a combination of anti-IgM and anti-CD40. Neither resting nor activated mouse T cells express OX-40L. The interaction of OX-40 (CD134) with OX-40L has been implicated in T cell-dependent humoral response. RM134L inhibits the costimulatory activity of mouse OX-40L transfected cells for anti-CD3-stimulated T cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IL2RA
Entrez