CD25/IL-2 R alpha Antibody (IL2R.1)

Images

 
Immunohistochemistry-Paraffin: CD25/IL-2 R alpha Antibody (IL2R.1) [NB600-564] - Human Tonsil
Immunohistochemistry-Paraffin: IL2RA/CD25 Antibody (IL2R.1) [NB600-564] - Formalin fixed paraffin embedded human tonsil stained with CD25, using ABC method.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC, IHC-Fr, IHC-P
Clone
IL2R.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CD25/IL-2 R alpha Antibody (IL2R.1) Summary

Immunogen
BALB/C mice were injected with recombinant human interleukin-2 receptor.
Localization
Cell Membrane
Specificity
This antibody is specific to a 55 kDa protein known as interleukin 2-receptor (IL-2R alpha). IL-2R alpha is over expressed on the surface of activated T cells, HTLV-1 or HTLV-2 positive T cell lines. It is moderately expressed on activated B cells, NK-cells, and macrophages.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IL2RA
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:25-1:75
Application Notes
Immunohistochemistry: use for 30 mins (ABC method) on pareffin-embedded sections. Use on frozen sections was reported in scientific literature (PMID: 22665223). Optimal dilution should be determined by the end user. Formalin fixed paraffin embedded sections require high temperature antigen retrieval unmasking with 10 mM citrate buffer, pH6.0 prior to staining. Has been reported to work in ICC/IF, mouse (PMID:22009917)
Publications
Read Publications using
NB600-564 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Purified immnoglobulin fraction in PBS with 2% BSA.
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for CD25/IL-2 R alpha Antibody (IL2R.1)

  • CD25 antigen
  • CD25
  • IDDM10
  • IL-2 R alpha
  • IL-2 receptor subunit alpha
  • IL2R alpha
  • IL-2R subunit alpha
  • IL2R
  • IL2RA
  • IL-2Ra
  • IL-2-RA
  • IL2-RA
  • interleukin 2 receptor, alpha
  • interleukin-2 receptor subunit alpha
  • p55
  • TAC antigen
  • TCGFR

Background

The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolyisis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD25/IL-2 R alpha Antibody (NB600-564)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-Fr, IHC-P.


Filter By Application
ICC/IF
(2)
IHC
(1)
IHC-Fr
(2)
IHC-P
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(2)
All Species

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol IL2RA
Entrez
Uniprot