BALB/C mice were injected with recombinant human interleukin-2 receptor.
This antibody is specific to a 55 kDa protein known as interleukin 2-receptor (IL-2R alpha). IL-2R alpha is over expressed on the surface of activated T cells, HTLV-1 or HTLV-2 positive T cell lines. It is moderately expressed on activated B cells, NK-cells, and macrophages.
Protein A or G purified
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Immunohistochemistry: use for 30 mins (ABC method) on pareffin-embedded sections. Use on frozen sections was reported in scientific literature (PMID: 22665223). Optimal dilution should be determined by the end user. Formalin fixed paraffin embedded sections require high temperature antigen retrieval unmasking with 10 mM citrate buffer, pH6.0 prior to staining. Has been reported to work in ICC/IF, mouse (PMID:22009917)
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
Purified immnoglobulin fraction in PBS with 2% BSA.
0.05% Sodium Azide
Protein A or G purified
Alternate Names for CD25/IL-2 R alpha Antibody (IL2R.1)
IL-2 R alpha
IL-2 receptor subunit alpha
IL-2R subunit alpha
interleukin 2 receptor, alpha
interleukin-2 receptor subunit alpha
The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolyisis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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