CD25/IL-2 R alpha Antibody (7D4)

Images

 
Flow Cytometry: CD25/IL-2 R alpha Antibody (7D4) [NBP1-27917] - Analysis using the PE conjugate of NBP1-27917. Cells from BALB/c spleen were activated by PMA (10 ng/ml) and ionomycin (400 ng/ml) for 48 hours, then ...read more
Flow Cytometry: CD25/IL-2 R alpha Antibody (7D4) [NBP1-27917] - Analysis using the Allophycocyanin conjugate of NBP1-27917. Staining of Con-A stimulated mouse splenocytes.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-27917
    • Availability
      Product Discontinued

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CD25/IL-2 R alpha Antibody (7D4) Summary

Immunogen
IL-2 dependent BALB/c helper T cell clone HT2
Specificity
Low affinity alpha chain IL-2 receptor (IL-2R alpha), Mr 55 kDa
Isotype
IgM Kappa
Clonality
Monoclonal
Host
Rat
Gene
IL2RA
Purity
IgM purified
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Applications/Dilutions

Dilutions
  • Blocking/Neutralizing 50-200 molar excess
  • Flow Cytometry 1:10 - 1:1000
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10 - 1:500

Reactivity Notes

Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
IgM purified

Alternate Names for CD25/IL-2 R alpha Antibody (7D4)

  • CD25 antigen
  • CD25
  • IDDM10
  • IL-2 R alpha
  • IL-2 receptor subunit alpha
  • IL2R alpha
  • IL-2R subunit alpha
  • IL2R
  • IL2RA
  • IL-2Ra
  • IL-2-RA
  • IL2-RA
  • interleukin 2 receptor, alpha
  • interleukin-2 receptor subunit alpha
  • p55
  • TAC antigen
  • TCGFR

Background

The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. (2) CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. (1, 3, 4) It is transiently expressed at a low level during B-cell development in bone marrow on the CD45R/B220 low TdT- sIg- pre-B II and CD45R/B220 low TdT- sIgM+sIgD- immature B cells, but not on the CD45R/B220 low TdT+ sIg- pro-B/pre-B I stage nor on CD45R/B220 high TdT- sIgM+sIgD+ mature B cells. (5, 6) CD25 is expressed at a higher level during very early T-cell development in fetal and adult thymus. (6, 7) The 7D4 monoclonal antibody reacts with an epitope between amino acids 125 and 212 that is distinct from that recognized by the monoclonal antibody 3C7. While the monoclonal antibody 7D4 does not block the binding of IL-2 to CD25, when used in combination with the monoclonal antibody 3C7 it results in higher levels of inhibition of IL-2-driven proliferation than either of these two monoclonal antibodies alone. (3, 8, 9)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IL2RA
Entrez