CD20 Antibody

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Western Blot: MS4A1/CD20 Antibody [NBP1-51905] - Analysis of CD20 in Human Lymph lysate (35ug protein in RIPA buffer) using NBP1-51905 at 0.003ug/ml. Primary incubated for 12 hours.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

CD20 Antibody Summary

Immunogen
Peptide with sequence CQDQESSPIENDSSP corresponding to C-Terminus according to NP_068769.2, NP_690605.1.
Epitope
CQDQESSPIENDSSP
Specificity
Please note that both reported variants (NP_068769.2 and NP_690605.1) encode the same protein.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MS4A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.03 - 0.1 ug/ml
  • Immunohistochemistry 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 35 kDa band observed in human lymph node lysates (calculated MW of 33.1 kDa band according to NP_068769.2). IHC-P: Human tonsil shows membranous staining mainly in the germinal center.
Publications
Read Publication using NBP1-51905.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note that both reported variants (NP_068769.2 and NP_690605.1) encode the same protein.

Alternate Names for CD20 Antibody

  • B1
  • B-lymphocyte antigen CD20
  • B-lymphocyte cell-surface antigen B1
  • B-lymphocyte surface antigen B1
  • Bp35
  • Bp35MGC3969
  • CD20 antigen
  • CD20 receptor
  • CD20
  • CD20S7
  • CVID5
  • LEU-16
  • Leukocyte surface antigen Leu-16
  • Ly-44
  • Membrane-spanning 4-domains subfamily A member 1
  • membrane-spanning 4-domains, subfamily A, member 1
  • MS4A1
  • MS4A2
  • S7

Background

CD20 is a B cell-specific 32kDa membrane protein involved in intracellular calcium regulation. CD20 expression begins after the induction of CD19 during development, and is coexpressed with IgM during the pre-B to immature B cell transition in bone marrow. Expression then increases during maturation with almost all mature cells expressing some level of CD20. The human CD20 antigen has proven to be an effective immunotherapeutic target in B cell lymphoma and several autoimmune diseases including rheumatoid arthritis and type I diabetes. CD20 consists of 4 transmembrane domains and a 44 amino acid extracellular loop domain where AISB12 binds.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD20 Antibody (NBP1-51905)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on CD20.

CD20 (Cluster of differentiation 20, Membrane-spanning 4-domains subfamily A member 1 (MS4A1), CVID5, B-lymphocyte surface antigen B1)
CD20 is a human B-lymphocyte surface molecule that spans the membrane four times and is expressed on both normal and malignant cells. The CD20 antigen displays a unique expression pattern among hematopoietic cells - it is present on human pre B-ly...  Read full blog post.

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Bioinformatics

Gene Symbol MS4A1
Entrez
Uniprot